ArticleScientific reports2026
Research and optimization of screening strategy for calcium-activated chloride channel modulators guided by electrophysiological characteristics.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Calcium-activated chloride channels (CaCCs) are essential for epithelial secretion, neuronal transmission, and smooth muscle function. Among the Anoctamin family, Anoctamin 1 (ANO1) and Anoctamin 2 (ANO2) are classical CaCCs proteins. ANO1 has been identified as a potential therapeutic target due to its involvement in diseases such as cancer and cystic fibrosis. However, current high-throughput screening (HTS) systems face limitations in achieving subtype-specific detection and optimizing screening strategies. Stable HTS cell models expressing ANO1 or ANO2 were constructed via lentiviral transduction in Fischer mouse thyroid (FRT) cells. The models were validated using flow cytometry, Reverse Transcription Polymerase Chain Reaction(RT-PCR), and YFP-H148Q/I152L-based iodide fluorescence quenching assays. Patch-clamp electrophysiology was employed to characterize ANO1 and ANO2 current properties. Although these electrophysiological features have been previously reported, their application in HTS workflows had not been systematically evaluated. ANO1 displayed notable current rundown under sustained stimulation with high Ca
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