Evidence map›Paper›PMID 41730830›Full record

ArticlePoultry science2026

Encapsulated Bacillus sp. THPS1, a novel thermophilic probiotic, enhances productivity and metabolic health in laying hens.

Waraphorn Sihamok, Orathai Dangsawat, Rachapong Sukhawong, Wasinee Boonamee, Kraibhumi Thongpan, Jessada Rattanawut, Rapeewan Sowanpreecha, Luu Tang Phuc Khang, Papungkorn Sangsawad, Nguyen Vu Linh and 2 more

Abstract read
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Dietary Encapsulation of a NovelAntioxidants (Basel, Switzerland) · 2026
    Article
  6. Coencapsulation of MultistrainInternational journal of microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Waraphorn SihamokDepartment of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand.
Orathai DangsawatScientific Laboratory and Equipment Center, Office of Surat Thani Campus, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand.
Rachapong SukhawongScientific Laboratory and Equipment Center, Office of Surat Thani Campus, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand.
Wasinee BoonameePSU. Witthayanusorn Suratthani School, Surat Thani 84000, Thailand.
Kraibhumi ThongpanPSU. Witthayanusorn Suratthani School, Surat Thani 84000, Thailand.
Jessada RattanawutDepartment of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand.
Rapeewan SowanpreechaDepartment of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand.
Luu Tang Phuc KhangAquatic Biotechnology Laboratory, Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
Papungkorn SangsawadSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Nguyen Vu LinhAquatic Biotechnology Laboratory, Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
Sk Injammul IslamCanadian River Institute, Department of Biology, University of Prince Edward Island.
Patima PermpoonpattanaDepartment of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand. Electronic address: patima.pe@psu.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Encapsulated probiotics, sustainable alternatives to antibiotic growth promoters in poultry production, enhance productivity and physiological health. Comparative evidence on strain-specific encapsulated Bacillus probiotics and their combined effects on production performance, egg quality, metabolic indicators, and hematological profiles in laying hens is insufficient. This study evaluated three encapsulated Bacillus strains (THPS1, PWR01, and OYNH19) for sporulation, encapsulation, and spore release efficiencies. A feeding trial in laying hens supplemented with 1 × 10⁶ spores/g feed was conducted over 4 weeks. Production performance, egg quality traits, serum lipid profiles, stress hormone levels, and hematological parameters were assessed. Sporulation and encapsulation efficiencies were consistently exceeded 96 %, while spore release efficiency exhibited significant variation across strains. OYNH19 demonstrated lower release efficiency compared to THPS1 and PWR01 strains (p = 0.042). Dietary supplementation with THPS1 significantly enhanced feed conversion ratio (FCR) (0.23 ± 0.01), resulting in a 23 % reduction compared to the control and other probiotic treatments (p = 0.001). Notably, egg production rate and body weight (BW) remained unchanged. However, egg weight (EW) was exhibited a significant increase in the THPS1 group by week 4 (65.22 ± 4.67 g; p = 0.041), without any adverse effects on eggshell or internal quality traits. Furthermore, THPS1 supplementation was associated with reduced serum triglyceride concentrations and distinct multivariate clustering, indicating coordinated alterations in production, metabolic, and hematological variables. Overall, encapsulated Bacillus sp. THPS1 demonstrated superior efficacy in optimizing feed efficiency while maintaining physiological stability.

Indexed as

BacillusChickensProbioticsReproductionAnimal FeedAnimalsDietDietary SupplementsFemaleBacillusEncapsulationGenomicsLaying henProbiotic

Identifiers

PMID41730830
PMCPMC12945637

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.