Evidence map›Paper›PMID 41728951›Full record

ArticleNucleic acids research2026

PolyP inhibits CobB deacetylase triggering a regulatory coupling to prevent DNA replication initiation.

Ewelina Boguszewska, Zuzanna Hirsz, Magdalena Sroka, Katarzyna Bury, Weronika Chmura, Agnieszka Strzałka, Marta Kołodziej, Jolanta Zakrzewska-Czerwińska, Igor Konieczny

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ewelina BoguszewskaLaboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. Abrahama 58, 80-307 Gdańsk, Poland.ORCID 0000-0003-1118-1831
Zuzanna HirszLaboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. Abrahama 58, 80-307 Gdańsk, Poland.ORCID 0000-0002-9331-3182
Magdalena SrokaLaboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. Abrahama 58, 80-307 Gdańsk, Poland.ORCID 0000-0003-0962-6707
Katarzyna BuryLaboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. Abrahama 58, 80-307 Gdańsk, Poland.ORCID 0000-0001-8197-1658
Weronika ChmuraLaboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. Abrahama 58, 80-307 Gdańsk, Poland.ORCID 0000-0002-6802-7766
Agnieszka StrzałkaDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław, ul. Joliot-Curie 14a, 50-383 Wrocław, Poland.ORCID 0000-0002-7092-0609
Marta KołodziejDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław, ul. Joliot-Curie 14a, 50-383 Wrocław, Poland.ORCID 0000-0001-6829-8737
Jolanta Zakrzewska-CzerwińskaDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław, ul. Joliot-Curie 14a, 50-383 Wrocław, Poland.ORCID 0000-0002-2042-974X
Igor KoniecznyLaboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. Abrahama 58, 80-307 Gdańsk, Poland.ORCID 0000-0002-1588-5601

Funding

National Science Centre 2021/43/B/NZ1/01360National Science Centre 25/597129/SPUB/SP/2024
6 · The paper itself

Abstract

Polyphosphate (polyP) is considered having regulatory functions in both procaryotic and eucaryotic cells. Under certain stress conditions, bacteria accumulate polyP, which results in liquid-liquid phase separation and polyP granules formation with not fully uncovered functions. We demonstrate that in starved Escherichia coli cells, replication initiator DnaA protein fails to form defined foci and does not bind to the origin of DNA replication (oriC), while to some extent interacts other sites on the chromosome. This is because polyP interacts with a long variant of CobB deacetylase and inhibits its activity, which results in an increased DnaA acetylation level preventing the DnaA interaction with oriC and consequently the initiation of DNA replication. This constitutes a polyP-dependent regulatory coupling targeting deacetylase for the inhibition of DNA replication initiation. Our experiments also demonstrate the importance of the multiplicity of regulatory mechanisms for the complete inhibition of initiation of DNA replication in stressed bacterial cells.

Indexed as

DNA ReplicationEscherichia coli ProteinsAcetylationBacterial ProteinsDNA-Binding ProteinsEscherichia coliOrigin Recognition ComplexReplication OriginBacterial ProteinsDnaA protein, BacteriaDNA-Binding ProteinsEscherichia coli ProteinsOriC chromosomal replication originOrigin Recognition Complex

Identifiers

PMID41728951
PMCPMC12926920

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.