Evidence map›Paper›PMID 41727169›Full record

ArticlebioRxiv : the preprint server for biology2026

Living Cells Employ Ubiquitin-Proteasomal System and Nucleotide Excision Repair Pathways to Remove Reactive Oxygen Species-Induced DNA-Protein Crosslinks (ROS-DPCs).

Cesar I Cyuzuzo, Monica Kruk, Qi Zhang, Duha Alshareef, James Harmon, Yuichi J Machida, Harrison W VanKoten, Swati S More, Colin Campbell, Natalia Y Tretyakova

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Cesar I CyuzuzoDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Monica KrukDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Qi ZhangDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Duha AlshareefDepartment of Pharmacology, University of Minnesota, Minneapolis, MN. 55455, USA.
James HarmonDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Yuichi J MachidaDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Harrison W VanKotenCenter for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
Swati S MoreDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Colin CampbellCenter for Drug Design, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.
Natalia Y TretyakovaDepartment of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-0621-6860

Funding

DNA Protein Cross-Links:Cellular Effects and Repair MechanismsR01ES023350 · NIEHS · UNIVERSITY OF MINNESOTA · PI CAMPBELL, COLIN R, TRETYAKOVA, NATALIA Y · 2014 to 2023
$5.0M
NIEHS NIH HHS R01 ES023350
6 · The paper itself

Abstract

Oxidative DNA damage caused by endogenous reactive oxygen species (ROS) is a key driver of mutagenesis, cellular dysfunction, and aging, contributing to diseases like cancer, neurodegeneration, rheumatoid arthritis, cardiovascular disorders, and diabetes. Although more than 20 oxidative base lesions have been identified, ROS-induced DNA-protein crosslinks (DPCs) are poorly characterized. ROS-DPCs are unusually bulky and highly toxic lesions that accumulate in metabolically active tissues with age, but their identities, biological consequences, and repair in living cells have remained elusive. In the present work, we characterized ROS-DPCs in human fibrosarcoma (HT1080) cells treated with hydrogen peroxide (H

Indexed as

DNA-protein crosslinksDNA repairproteasomal degradationReactive oxygen species

Identifiers

PMID41727169
PMCPMC12919063

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.