Evidence map›Paper›PMID 41727167›Full record

ArticlebioRxiv : the preprint server for biology2026

HAPI Cells are SIM-A9-related Mouse Microglial Cells Useful for

Ryan P Mayers, Sausan M Jaber, Nicolas Verhoeven, Ayodele Jaiyesimi, Brian M Polster

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ryan P MayersDepartment of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID 0000-0002-5627-9800
Sausan M JaberDepartment of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID 0000-0002-5247-0560
Nicolas VerhoevenProgram in Molecular Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID 0009-0009-2936-4485
Ayodele JaiyesimiDepartment of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research, University of Maryland School of Medicine, Baltimore, MD, USA.
Brian M PolsterDepartment of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID 0000-0003-2571-7510

Funding

TRAINING PROGRAM IN INTEGRATIVE MEMBRANE BIOLOGYT32GM008181 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI RIZZO, MEGAN A, TRUDEAU, MATTHEW C · 1987 to 2022
$4.9M
Reprogramming proinflammatory microglia by restoring mitochondrial functionR01NS112212 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI POLSTER, BRIAN M · 2020 to 2024
$2.9M
Targeting mitochondrial Complex I in neonatal hypoxia-ischemiaR01NS122777 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI BRIAN M POLSTER, JAYLYN Waddell WADDELL · 2022 to 2026
$2.5M
Novel Mechanisms of Microglial Neurotoxicity at Physiological OxygenR01NS085165 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI POLSTER, BRIAN M · 2013 to 2017
$1.7M
Advanced components for an existing spinning disk confocal in an established core imaging facilityS10OD026698 · OD · UNIVERSITY OF MARYLAND BALTIMORE · PI BLANPIED, THOMAS A · 2019 to 2019
$274k
NIGMS NIH HHS T32 GM008181NIH HHS S10 OD026698NINDS NIH HHS R01 NS085165NINDS NIH HHS R01 NS112212NINDS NIH HHS R01 NS122777
6 · The paper itself

Abstract

Highly aggressively proliferating immortalized (HAPI) cells were initially described as a spontaneously immortalized rat cell line isolated from a mixed neonatal rat glial population. It was demonstrated that HAPI cells are phagocytic, stain for macrophage-/microglia-specific markers like CD11b and GLUT5, and exhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) release. These characteristics led to their widespread use as a rat microglial cell line. Here, we report that HAPI cells are mouse cells, not rat cells, but further establish that they have a microglia-like identity and properties useful for

Indexed as

interferon-alphainterferon-gammalipopolysaccharidemicrogliaMitochondrianitric oxideTMEM119

Identifiers

PMID41727167
PMCPMC12918914

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.