Evidence map›Paper›PMID 41726963›Full record

ArticlebioRxiv : the preprint server for biology2026

Membrane Kymograph Generator: A cross-platform GUI software for automated generation and analysis of kymographs along dynamic cell boundaries.

Tatsat Banerjee, Bedri Abubaker-Sharif, Peter N Devreotes, Pablo A Iglesias

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Tatsat BanerjeeDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.ORCID 0000-0002-3490-3959
Bedri Abubaker-SharifDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.ORCID 0000-0001-5003-7934
Peter N DevreotesDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.ORCID 0000-0002-3260-1455
Pablo A IglesiasDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.ORCID 0000-0002-0840-155X

Funding

Excitable Networks in Directed Cell MigrationR35GM118177 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Peter N Devreotes · 2016 to 2026
$12.1M
Molecular Mechanisms of Cytoskeletal Mechanosensory SystemsR01GM149073 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Pablo A. Iglesias, DOUGLAS N ROBINSON · 2023 to 2026
$2.6M
Zeiss LSM780 Confocal Microscope for a Core FacilityS10OD016374 · OD · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2013 to 2013
$584k
NIGMS NIH HHS R01 GM149073NIGMS NIH HHS R35 GM118177NIH HHS S10 OD016374
6 · The paper itself

Abstract

Summary: The plasma membrane and accompanying cortex serve as one of the major hubs of the signal transduction and cytoskeletal activities that collectively regulate numerous cell physiological processes such as migration, polarity, macropinocytosis, phagocytosis, cytokinesis, etc. Yet, dynamically tracking membrane-cortex associated protein or lipid kinetics over time from live-cell image series remains a challenging task, primarily due to the difficulty of accurately extracting and aligning the cell boundary between consecutive frames, as the cell continuously deforms and moves. Here, we present Availability and Implementation: Membrane Kymograph Generator is a free and open-source software, licensed under GNU General Public License 3.0 or later. This software is cross-platform: It can be graphically installed on both x86-64 and AArch64/ARM64 computers, running either Windows, macOS, or any standard Linux distribution. The software is distributed as single installer files (and portable executables) targeting specific hardware architectures and operating systems, and hence, it can be installed natively without any dependency resolution. The source code, detailed documentation, specific installers, portable binaries, and test data are freely available at https://github.com/tatsatb/membrane-kymograph-generator. Additionally, since the software is written in Python, it can also be installed inside any Python environment using PIP package manager (package ID: https://pypi.org/project/membrane-kymograph) and can also be interacted via a built-in Python API.

Identifiers

PMID41726963
PMCPMC12919045

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.