ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
Deep Volumetric Super-Resolution Imaging in Thick Biological Specimens With Sparse Scanning SIM.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
High-resolution fluorescence microscopy deep inside thick, live specimens remains a major challenge due to scattering and limited penetration depth. Structured illumination microscopy (SIM) is widely used for fast super-resolution imaging, yet its sensitivity to scattering strongly restricts volumetric applications. Here we present sparse scanning SIM (SS-SIM), a variant for deep imaging that integrates rapid laser beam scanning with pixel-addressed intensity modulation and sCMOS detection. SS-SIM operates with either one- or two-photon excitation and achieves a spatial resolution of ∼150 nm, and maintains a 1.6/1.7-fold (lateral/axial) resolution enhancement over wide-field microscopy throughout the available imaging depth limited by the working distances of our water-immersion objectives (300/600 µm), enabling volumetric visualization of dense biological samples. By combining simplicity, robustness, and super-resolution performance, SS-SIM expands the applicability of SIM to thick tissues, organoids, and small organisms, providing a practical route to high-contrast volumetric imaging in complex biological environments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.