ArticlePlant molecular biology2026
Characterization of an (E, E)-farnesyl pyrophosphate synthase in Saxifraga stolonifera involved in triterpenoid biosynthesis.
Article in Plant molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Saxifraga stolonifera is a Chinese traditional medicine for otitis media treatment. A few terpenoids were identified from S. stolonifera but the involved genes are unclear. Here, we mined a farnesyl pyrophosphate synthase gene (SsFPS) from the full-length transcriptome of S. stolonifera and confirmed it was expressed in cytoplasm. Subsequently, the enzymatic activity of SsFPS was characterized by in-vitro assays and heterologous expression in E.coli. Moreover, the gene expression pattern of SsFPS was positively correlated to which of a squalene synthase gene (SsSS) involved in triterpenoid biosynthesis and consistent to the accumulation pattern of total saponins in tissues. Taken together, SsFPS was characterized as an (E, E)-farnesyl pyrophosphate synthase in S. stolonifera and suggested to participate in sesquiterpenoid and triterpenoid biosynthesis which provides the support to explore the biosynthesis and pharmacological activity of triterpenoid in Saxifraga.
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