Evidence map›Paper›PMID 41722571›Full record

ArticleCell metabolism2026

Modeling lipid homeostasis using stable isotope tracing and flux analysis.

Karl Wessendorf-Rodriguez, Maureen L Ruchhoeft, Christopher W Murray, Yale Huang, Ethan L Ashley, Hector M Galvez, Grace H McGregor, Shrikaar Kambhampati, Reuben J Shaw, Christian M Metallo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell metabolism, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Karl Wessendorf-RodriguezDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Maureen L RuchhoeftDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA.
Christopher W MurrayDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA.
Yale HuangDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Ethan L AshleyDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA.
Hector M GalvezDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA.
Grace H McGregorDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA.
Shrikaar KambhampatiDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA.
Reuben J ShawDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Christian M MetalloDepartment of Molecular and Cell Biology, the Salk Institute for Biological Studies, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA. Electronic address: metallo@salk.edu.

Funding

Viral Vector Core (VVC)P30CA014195 · NCI · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI Reuben Shaw · 1985 to 2026
$82.8M
Decoding And Targeting The LKB1-AMPK Signaling Pathway In CancerR35CA220538 · NCI · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI Reuben Shaw · 2017 to 2026
$11.3M
Non-essential amino acids and sphingolipid diversity in cancer progressionR01CA234245 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI METALLO, CHRISTIAN MICHAEL · 2019 to 2023
$1.9M
NCI NIH HHS P30 CA014195NCI NIH HHS R01 CA234245NCI NIH HHS R35 CA220538
6 · The paper itself

Abstract

Lipids enable compartmentation and coordinate membrane-localized signaling events in cells, and dysregulation of lipid metabolism is linked to many disease states. However, limited tools are available for quantifying metabolic fluxes across the lipidome. To measure fluxes encompassing lipid homeostasis in cells and tissue slices, we apply stable isotope tracing, liquid chromatography-high-resolution mass spectrometry, and network-based isotopologue modeling to non-small cell lung cancer (NSCLC) models. Lipid metabolic flux analysis (Lipid-MFA) enables quantitation of fatty acid synthesis, elongation, headgroup assembly, and salvage reactions within virtually any biological system. Using Lipid-MFA, we observed decreased fatty acid synthase and very long-chain fatty acid (VLCFA) elongation fluxes, along with increased sphingolipid recycling, in p53-deficient versus liver kinase B1 (LKB1)-deficient NSCLC tumors using precision-cut lung slice culture. We also apply Lipid-MFA to demonstrate the unique trafficking of ceramides with distinct n-acyl chain lengths, highlighting the utility of this approach in elucidating molecular mechanisms in lipid homeostasis.

Indexed as

Carcinoma, Non-Small-Cell LungHomeostasisLipid MetabolismModels, BiologicalAnimalsCeramidesFatty AcidsHumansIsotope LabelingLung NeoplasmsMiceSphingolipidsCeramidesFatty AcidsSphingolipidsceramideELOVL1lipid homeostasisLKB1metabolic flux analysisnon-small cell lung cancerprecision-cut lung slice culturesphingolipidsTP53very long-chain fatty acids

Identifiers

PMID41722571
PMCPMC13477146

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.