Evidence map›Paper›PMID 41721424›Full record

ArticleGenome biology2026

Decoding human tRNA modifications and crosstalk by enhanced single-read analysis.

Mahdi Assari, Brandon J Chew, Mohammad Amin Bayat Tork, Marek Sobczyk, Chenyou Zhu, Hoang Anh V Tran, Dominika Rudzka, Wen Zhang, Tao Pan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mahdi AssariDepartments of Chemistry, University of Chicago, Chicago, IL, 60637, USA.
Brandon J ChewMolecular Genetics and Cell Biology, University of Chicago, Chicago, IL, 60637, USA.
Mohammad Amin Bayat TorkBiochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA.
Marek SobczykBiochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA.
Chenyou ZhuDepartments of Chemistry, University of Chicago, Chicago, IL, 60637, USA.
Hoang Anh V TranBiochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA.
Dominika RudzkaBiochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA.
Wen ZhangBiochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA.
Tao PanBiochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA. taopan@uchicago.edu.

Funding

Transfer RNA sequencing and application to cancer research and clinicsR33CA272357 · NCI · UNIVERSITY OF CHICAGO · PI BISSONNETTE, BRUCE MARC, PAN, TAO · 2022 to 2024
$1.2M
NIH HHS R33CA272357
6 · The paper itself

Abstract

backgroundTransfer RNA (tRNA) modifications play important roles in regulating gene expression and protein synthesis, yet their dynamic interplay remains elusive. The human tRNAome contains approximately 40 modification types, distributed on average at 13 sites in cytosolic tRNAs and 6 sites in mitochondrial tRNAs. tRNA modifications exhibit dynamic patterns dependent on cell types and cell states, underscoring the need for advancing methodologies to assess their variations and crosstalk transcriptome-wide.

resultsHere, we develop enhanced single-read analysis of tRNA crosstalks (eSLAC), an integrative platform combining multiplex small RNA sequencing (MSR-seq) with expanded detection of pseudouridine (Ψ), 5-formylcytidine (f

conclusionsThis study establishes a framework for dissecting the interconnected architecture of tRNA modome and its functional complexity.

Indexed as

RNA Processing, Post-TranscriptionalRNA, TransferCytidineHumansPseudouridineSequence Analysis, RNA5-formylcytidineCytidineN-acetylcytidinePseudouridineRNA, TransferCrosstalkModificationPseudouridineSingle-read data analysisTRNA

Identifiers

PMID41721424
PMCPMC13023127

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.