ArticleBMC medical genomics2026
Hub genes and molecular mechanisms related to hypospadias based on transcriptome sequencing data and bioinformatics analysis.
Article in BMC medical genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHypospadias, a prevalent congenital anomaly affecting patients’ quality of life, remains incompletely understood in terms of its pathogenesis. This study aimed to identify key genes and molecular mechanisms through transcriptome sequencing and bioinformatics analysis.
methodsTranscriptome sequencing was conducted on foreskin samples from 15 patients with hypospadias and 15 controls. Differential expression analysis and WGCNA were used to identify differentially expressed genes (DEGs) and key modules. Genes common to both approaches were further analyzed through PPI network filtering, followed by feature selection using LASSO and SVM-RFE algorithms. Hub genes were identified based on an AUC > 0.8. Functional, immune, and regulatory analyses were performed to investigate their biological roles.
resultsThree hub genes—CSF3R, SELL, and FCN1—demonstrated significant diagnostic potential (AUC > 0.8). Functional enrichment analysis revealed their association with cytokine receptor interaction and chemokine signaling pathways. Eleven immune cell types, including activated dendritic cells, were found to be elevated in the patient cohort. All hub genes were upregulated in the disease group.
conclusionCSF3R, SELL, and FCN1 represent potential diagnostic biomarkers for hypospadias and provide valuable insights into its molecular mechanisms and potential therapeutic approaches.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.