Evidence map›Paper›PMID 41721220›Full record

ArticleBMC microbiology2026

Mechanisms of constipation alleviation by Lacticaseibacillus paracasei BGI-N2: insights from genes to phenotypes.

Xing Rao, Qiang Luo, Wanting Wei, Benliang Wei, Xinyu Yang, Yanhong Liu, Yangfeng Wen, Zhihui Ma, Zhinan Wu, Haifeng Zhang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xing Rao *State Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen 518083, China.
Qiang Luo *BGI Precision Nutrition, Shenzhen, 518083, China.
Wanting WeiBGI Research, Shenzhen, 518083, China.
Benliang WeiBGI Precision Nutrition, Shenzhen, 518083, China.
Xinyu YangState Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen 518083, China.
Yanhong LiuBGI Precision Nutrition, Shenzhen, 518083, China.
Yangfeng WenState Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen 518083, China.
Zhihui MaBGI Precision Nutrition, Shenzhen, 518083, China.
Zhinan WuState Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen 518083, China.
Haifeng ZhangBGI Precision Nutrition, Shenzhen, 518083, China.
Liang XiaoState Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen 518083, China.
Yiyi ZhongBGI Precision Nutrition, Shenzhen, 518083, China. zhongyiyi@genomics.cn.
Yuanqiang ZouState Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen 518083, China. zouyuanqiang@genomics.cn.

Funding

the Shenzhen Municipal Government of China KCXFZ20240903094006009
6 · The paper itself

Abstract

The global prevalence of constipation has been increasing in recent years, posing an urgent need for safer and more effective therapeutic strategies. Given the favorable safety profile and beneficial effects on gut health, probiotics have emerged as a promising means for improving constipation. This study investigated the probiotic potential of Lacticaseibacillus paracasei BGI-N2 (BGI-N2) using integrated genomic and phenotypic analyses. The complete genome of BGI-N2 comprised 3,102,337 bp with a 46.4% GC content and 2,937 coding sequences, including multiple genes related to carbon source fermentation, gastrointestinal tolerance, and antioxidant activities. Furthermore, genomic analysis confirmed the favorable safety profile of BGI-N2. In vitro, this strain showed robust tolerance to extreme gastrointestinal conditions and significant inhibition against common enteropathogens. In constipated zebrafish, it enhanced motility by increasing motilin (MTL) and 5-hydroxytryptamine (5-HT) while decreasing nitric oxide levels. Mechanistically, BGI-N2 upregulated kitla, kitlb, and kitb genes to promote Cajal interstitial cell development, while enhancing 5-HT accumulation through coordinated regulation of biosynthetic genes (tph1a, tph1b, tph2) and the transporter gene (sert). This study provides comprehensive genomic insights for BGI-N2 while establishing a theoretical foundation for understanding host-microbe interactions in intestinal motility disorders.

Indexed as

ConstipationLacticaseibacillus paracaseiProbioticsAnimalsGenome, BacterialMotilinPhenotypeSerotoninZebrafishMotilinSerotonin5-hydroxytryptamineComplete genomeConstipationLacticaseibacillus paracaseiZebrafish

Identifiers

PMID41721220
PMCPMC13032640

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.