The Ies6 subunit is essential for INO80-mediated nucleosome organization.

Ashish Kumar Singh, Felix Mueller-Planitz

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

2 authors.

Ashish Kumar SinghInstitute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307, Dresden, Germany.

Felix Mueller-PlanitzInstitute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307, Dresden, Germany. felix.mueller-planitz@tu-dresden.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ATP-dependent nucleosome remodelers of the INO80 family regulate chromatin by sliding, spacing and positioning nucleosomes. The INO80 remodeler is organized into structural modules that regulate its remodeling activity. Here, we investigate the role of the Ies6 subunit within the Arp5-Ies6 module towards nucleosome positioning and spacing in Saccharomyces cerevisiae. We show that Ies6 is critical for establishing genome-wide nucleosome organization. Deletion of IES6 reduces nucleosome spacing by 3 bp and disrupts regular nucleosome arrays across most genes. Surprisingly, deletion of IES6 is synthetically lethal with the deletion of ISW2, a remodeler from the ISWI family, indicating functional redundancy in nucleosome organization. Notably, INO80 binding directly predicts the role of Ies6 in INO80-mediated nucleosome organization, whereas changes in gene expression do not correlate with altered nucleosome spacing or array regularity. Together, our results highlight the essential role of the Ies6 subunit in INO80-mediated chromatin organization.

Indexed as

NucleosomesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAdenosine TriphosphatasesChromatin Assembly and DisassemblyDNA-Binding ProteinsGene Expression Regulation, FungalProtein SubunitsTranscription FactorsAdenosine TriphosphatasesDNA-Binding ProteinsINO80 complex, S cerevisiaeNucleosomesProtein SubunitsSaccharomyces cerevisiae ProteinsTranscription FactorsIes6INO80NRLNucleosome positioning and spacingNucleosome remodeler

Identifiers

PMID41720950

DOI10.1038/s41598-026-40504-8

PMCPMC12929563

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.