Evidence map›Paper›PMID 41720800›Full record

ArticleScientific reports2026

Involvement of AKT/β-catenin in Enhanced Osteogenesis by Cannabidiol in Bone Stromal Cells Harvested from Human Tori.

Anupong Makeudom, Mananchaya Taechukorn, Kanyanut Muenhong, Pantira Danvirunhavanit, Janthima Petchnuan, Ekapong Dechtham, Pansakorn Sangkaew, Sumit Suamphan, Suttichai Krisanaprakornkit

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Phytocannabinoids and bone health.Current osteoporosis reports · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Anupong MakeudomSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Mananchaya TaechukornSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Kanyanut MuenhongSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Pantira DanvirunhavanitSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Janthima PetchnuanSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Ekapong DechthamSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Pansakorn SangkaewSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Sumit SuamphanOrthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand.
Suttichai KrisanaprakornkitSchool of Dentistry, Mae Fah Luang University, Chiang Rai, 57100, Thailand. suttichai.kri@mfu.ac.th.

Funding

Research Grant for New Scholar, National Research Council of Thailand (NRCT) N42A660843
6 · The paper itself

Abstract

Cannabidiol (CBD), a major cannabinoid of Cannabis sativa, has been utilized for several medical purposes, such as, an anti-resorptive activity that may benefit treatment of alveolar bone destruction in periodontitis. This study aimed to investigate osteogenic effects of CBD in primary bone stromal cells harvested from jaw tori of healthy patients. To determine cytotoxicity of CBD, the bone stromal cells were treated with various doses of CBD for 24 or 48 h and then analyzed by an alamarBlue® assay. No cytotoxicity was found in these cells treated with CBD up to 10 µM. Enhanced osteogenic differentiation and biomineralization of the cells treated with non-toxic doses of CBD were determined by alkaline phosphatase staining and Alizarin Red and von Kossa staining, respectively, and confirmed by upregulated mRNA expressions of runt-related transcription factor 2 (RUNX2), bone sialoprotein (BSP), and Osterix. Treatment with CBD significantly enhanced osteogenic differentiation and biomineralization and upregulated mRNA expressions of RUNX2, BSP, and Osterix (p < 0.05). Pretreatment with MK-2206, an AKT inhibitor, or with CWP232228, a β-catenin antagonist, significantly decreased the increased Alizarin Red staining and the upregulated expressions of BSP and Osterix (p < 0.05), suggesting involvement of AKT/β-catenin in osteogenic induction upon treatment with CBD.

Indexed as

beta CateninCannabidiolMesenchymal Stem CellsOsteogenesisProto-Oncogene Proteins c-aktCalcification, PhysiologicCell DifferentiationCells, CulturedCore Binding Factor Alpha 1 SubunitHumansIntegrin-Binding Sialoproteinbeta CateninCannabidiolCore Binding Factor Alpha 1 SubunitCTNNB1 protein, humanIntegrin-Binding SialoproteinProto-Oncogene Proteins c-aktAk strain transforming (AKT)CannabidiolOsteoblastOsteogenesisβ-catenin

Identifiers

PMID41720800
PMCPMC13009283

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.