Evidence map›Paper›PMID 41719393›Full record

ArticleScience advances2026

Glomerular routing of tumor-derived extracellular vesicles substantiates urinary biopsy.

Shota Kawaguchi, Taiga Ajiri, Rina Mitsuya, Reiko Tsuchiya, Koki Kunitake, Yoshikazu Tanaka, Takeshi Yokoyama, Kiichi Sato, Yusuke Sato, Zetao Zhu and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Shota KawaguchiDepartment of Life Science and Technology, Institute of Science Tokyo, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan.ORCID 0009-0000-8341-3916
Taiga AjiriDepartment of Life Science and Technology, Institute of Science Tokyo, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan.ORCID 0000-0002-9019-9935
Rina MitsuyaDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.ORCID 0009-0008-8939-1699
Reiko TsuchiyaGraduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Koki KunitakeGraduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.ORCID 0009-0006-0398-2267
Yoshikazu TanakaGraduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Miyagi, Japan.ORCID 0000-0002-7893-3236
Takeshi YokoyamaGraduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Miyagi, Japan.ORCID 0000-0002-0299-9140
Kiichi SatoGraduate School of Science and Technology, Gunma University, Tenjin-cho, Kiryu 376-8515, Gunma, Japan.
Yusuke SatoDepartment of Chemistry, Graduate School of Science, Tohoku University, 6-3 Aramaki Aza-Aoba, Aoba-ku, Sendai 980-8578, Miyagi, Japan.ORCID 0000-0002-5824-1654
Zetao ZhuDepartment of Life Science and Technology, Institute of Science Tokyo, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan.ORCID 0000-0001-6156-748X
Kunanon ChattrairatDepartment of Life Science and Technology, Institute of Science Tokyo, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan.ORCID 0000-0001-7587-6777
Yasuko KobayashiDepartment of Pediatrics, Graduate School of Medicine, Gunma University, 3-39-22 Showa-machi, Maebashi 371-8511, Gunma, Japan.
Kimiko InoueDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Hokkaido, Japan.
Keisuke ImaedaDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Hokkaido, Japan.ORCID 0000-0001-8877-1085
Kosei UenoDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Hokkaido, Japan.ORCID 0000-0002-4882-7854
Sou RyuzakiDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Hokkaido, Japan.
Akira KatoThe Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Yasuyuki KimuraDepartment of Clinical and Experimental Neuroimaging, Center for Development of Advanced Medicine for Dementia, Research Institute, National Center for Geriatrics and Gerontology, Obu 474-8511, Japan.ORCID 0000-0002-7927-9483
Atsushi NatsumeThe Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Ryosuke KojimaGraduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.ORCID 0000-0002-3792-9222
Takao YasuiDepartment of Life Science and Technology, Institute of Science Tokyo, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan.ORCID 0000-0003-0333-3559

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Urinary small extracellular vesicles (sEVs), which can reflect systemic conditions, hold great promise for noninvasive cancer diagnostics, yet the mechanism by which tumor-derived sEVs reach urine remains unclear. Here, we demonstrate that the glomerulus actively transcytoses circulating tumor-derived sEVs into urine. Using CRISPR guide RNA-tagged glioma sEVs and bioluminescent/fluorescent green-enhanced nano-lantern (GeNL)-tagged lung and pancreatic cancer sEVs, we tracked their journey from tumors to urine in multiple mouse models. In vivo and in vitro analyses revealed endocytic uptake and transcytotic release by glomerular cells, accompanied by changes in sEV size and surface composition. GeNL-tagged sEVs consistently showed higher signals in urine than plasma, indicating selective excretion. These findings redefine the glomerulus as a dynamic regulator of sEV processing and establish a mechanistic foundation for urinary liquid biopsy.

Indexed as

Extracellular VesiclesKidney GlomerulusNeoplasmsAnimalsBiopsyCell Line, TumorHumansLiquid BiopsyMice

Identifiers

PMID41719393
PMCPMC12922736

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.