Protocol for modeling innate immune training by repeated alum exposure in mice (adjuvant conditioning).

Thais Boccia, Matheus Deroco Veloso da Silva, Victor Fattori, Weikang Pan, Michael S Rogers, Alex G Cuenca

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Thais BocciaDepartment of Surgery, Boston Children's Hospital - Harvard Medical School, Boston, MA 02115, USA.

Matheus Deroco Veloso da SilvaLaboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Immunology, Parasitology and General Pathology, Londrina State University, Londrina, PR 86.057-970, Brazil.

Victor FattoriVascular Biology Program, Department of Surgery, Boston Children's Hospital -Harvard Medical School, Boston, MA 02115, USA.

Weikang PanDepartment of Surgery, Boston Children's Hospital - Harvard Medical School, Boston, MA 02115, USA.

Michael S RogersVascular Biology Program, Department of Surgery, Boston Children's Hospital -Harvard Medical School, Boston, MA 02115, USA.

Alex G CuencaDepartment of Surgery, Boston Children's Hospital - Harvard Medical School, Boston, MA 02115, USA. Electronic address: alex.cuenca@childrens.harvard.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a protocol to model the effects of innate immune training and reprogramming through repeated alum exposure in mice, enabling the evaluation of immunoregulatory cell populations in both steady-state and inflammatory conditions. We describe steps for integrating adoptive cell transfer, in vivo antigen-specific immunization, and transplant models. We also detail procedures for in vitro stimulation of human peripheral blood mononuclear cells (PBMCs) to explore translational relevance. For complete details on the use and execution of this protocol, please refer to Boccia et al.

Indexed as

Adjuvants, ImmunologicAlum CompoundsImmunity, InnateAnimalsHumansLeukocytes, MononuclearMiceTrained ImmunityAdjuvants, ImmunologicAlum Compoundsaluminum sulfateCell-based AssaysCell cultureCell isolationFlow CytometryImmunology

Identifiers

PMID41719134

DOI10.1016/j.xpro.2026.104390

PMCPMC12936741

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.