Evidence map›Paper›PMID 41719132›Full record

ArticleSTAR protocols2026

Protocol for isolating plant-derived extracellular vesicles.

Yunyi Chen, Le Xu, Yuanyuan Yu, Zhifeng Xu, Songhua Xiao, Hongcheng Mai

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yunyi ChenDepartment of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, China.
Le XuDepartment of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, China.
Yuanyuan YuCollege of Pharmacy, Jinan University, Guangzhou, Guangdong 510000, China.
Zhifeng XuDepartment of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, China.
Songhua XiaoDepartment of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, China. Electronic address: xiaosh@mail.sysu.edu.cn.
Hongcheng MaiDepartment of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, China. Electronic address: maihch7@mail.sysu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant-derived extracellular vesicles (PEVs) are nanosized, lipid bilayer-enclosed vesicles secreted by plant cells into the extracellular space, playing critical roles in intercellular communication. Here, we present a comprehensive and reproducible protocol for isolating PEVs from diverse plant species. Our integrated approach combines differential centrifugation, ultrafiltration, ultracentrifugation, and sucrose density gradient centrifugation to achieve efficient enrichment and purification of PEVs while effectively removing cellular debris. Finally, we characterize the isolated PEVs in terms of morphology, particle size distribution, and zeta potential.

Indexed as

Cell FractionationExtracellular VesiclesPlant CellsPlantsCentrifugation, Density GradientUltracentrifugationUltrafiltrationBiotechnology and bioengineeringCell BiologyPlant sciences

Identifiers

PMID41719132
PMCPMC12934269

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.