Evidence map›Paper›PMID 41719131›Full record

ArticleSTAR protocols2026

Protocol to establish glioma stem cell spheroids and an enriched cancer-associated fibroblast population from a single patient tumor specimen.

Caroline Delmas, Elisabeth Cohen-Jonathan-Moyal, Catherine Seva

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Caroline DelmasCentre de Recherche en Cancérologie de Toulouse (CRCT), INSERM U1037, Université de Toulouse, 31000 Toulouse, France; IUCT-Oncopole, Oncopole Claudius Regaud, 31000 Toulouse, France. Electronic address: delmas.caroline@iuct-oncopole.fr.
Elisabeth Cohen-Jonathan-MoyalCentre de Recherche en Cancérologie de Toulouse (CRCT), INSERM U1037, Université de Toulouse, 31000 Toulouse, France; IUCT-Oncopole, Oncopole Claudius Regaud, 31000 Toulouse, France.
Catherine SevaCentre de Recherche en Cancérologie de Toulouse (CRCT), INSERM U1037, Université de Toulouse, 31000 Toulouse, France. Electronic address: cathy.seva@inserm.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glioblastoma is an aggressive, therapy-resistant brain tumor in which the role of cancer-associated fibroblasts (CAFs) remains poorly defined. We present a protocol to simultaneously establish glioblastoma stem cell (GSCs) spheroids and CAFs from a single patient specimen. We describe steps to dissociate tumor tissue, generate a single-cell suspension, and establish GSCs spheroids and CAFs cultures. This protocol details CAFs enrichment, passaging, phenotypic characterization, and cryopreservation. For complete details on the use and execution of this protocol, please refer to Delmas et al.

Indexed as

Brain NeoplasmsCancer-Associated FibroblastsCell Culture TechniquesGlioblastomaGliomaNeoplastic Stem CellsSpheroids, CellularCryopreservationHumansCancerCell cultureCell isolationStem Cells

Identifiers

PMID41719131
PMCPMC12934319

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.