Evidence map›Paper›PMID 41718830›Full record

ArticlePediatric surgery international2026

Oesophageal tissue engineering: optimisation of stereotactic robotic cell injection in decellularised oesophageal scaffolds.

Koji Yamada, Julia Perea Paizal, Elena Canovai, Casper Orens, Ilaria Marcoccio, Natalie Durkin, Lorenzo Caciolli, Satoshi Ieiri, Simon Eaton, Sara Mantero and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pediatric surgery international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Koji YamadaStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Julia Perea PaizalStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Elena CanovaiStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Casper OrensStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Ilaria MarcoccioStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Natalie DurkinStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Lorenzo CaciolliStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Satoshi IeiriDepartment of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Kagoshima, Japan.
Simon EatonStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Sara ManteroDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Milan, Italy.
Paolo De CoppiStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK. p.decoppi@ucl.ac.uk.
Marco PellegriniStem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK. marco.pellegrini@ucl.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeOesophageal substitution following atresia repair, caustic damage or cancer of the oesophagus can be challenging. We and others are working on engineering oesophageal tissue using a combination of decellularised oesophagi and cell injection. So far this has been achieved using highly operator-dependent techniques. This study aimed to establish a reproducible method for cell delivery into scaffolds.

methodsTo improve consistency, a stereotaxic robotic platform was adapted to deliver a suspension of porcine gelatin and cells in a 1:1 ratio. The scaffold was mounted on a 3D-printed rod linked to a stepper motor, enabling automated 36° rotation for circumferential coverage. Two circumferential rows, each rotated 36°, with 3 - 2 points at 3-mm intervals, ensured even seeding. Injection depth was calibrated to target the inner layer.

resultsCells injected robotically remained viable, with no significant difference from manual injection. Post-injection analyses confirmed cell viability and distribution within the scaffold.

conclusionAutomated robotic injection provides a reliable, reproducible alternative to manual methods, reducing operator bias.

Indexed as

Esophageal AtresiaEsophagusRoboticsRobotic Surgical ProceduresStereotaxic TechniquesTissue EngineeringTissue ScaffoldsAnimalsHumansSwineOesophageal atresiaRecellularisationRobotic injectionTissue clearingTissue engineering

Identifiers

PMID41718830
PMCPMC12923411

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.