Evidence map›Paper›PMID 41716354›Full record

ArticleF1000Research2025

A guide to selecting high-performing antibodies for Optineurin (UniProt ID: Q96CV9) for use in western blot, immunoprecipitation, and immunofluorescence.

Vera Ruíz Moleón, Riham Ayoubi, Charles Alende, Maryam Fothouhi, Joel Ryan, Sara González Bolívar, Donovan Worrall, Thomas M Durcan, Claire M Brown, Vincent Francis and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in F1000Research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Vera Ruíz MoleónNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.ORCID https://orcid.org/0000-0003-3728-3158
Riham AyoubiNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.
Charles AlendeNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.ORCID https://orcid.org/0009-0005-4611-6134
Maryam FothouhiNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.
Joel RyanAdvanced BioImaging Facility, McGill University, Montreal, Québec, Canada.
Sara González BolívarNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.ORCID https://orcid.org/0000-0002-4299-8281
Donovan WorrallNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.ORCID https://orcid.org/0000-0001-5676-5473
Thomas M DurcanNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.
Claire M BrownAdvanced BioImaging Facility, McGill University, Montreal, Québec, Canada.
Vincent FrancisNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.ORCID https://orcid.org/0009-0000-7535-8718
Peter S McPhersonNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.
Carl LaflammeNeuroscience and Neurosurgery, McGill University, Montreal, Québec, Canada.ORCID https://orcid.org/0000-0001-5906-025X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optineurin (OPTN) is a multifunctional cytoplasmic adaptor protein implicated in maintaining neuronal homeostasis through its roles in selective autophagy, vesicle trafficking, and regulation of inflammatory signaling. Mutations in the

Indexed as

AntibodiesBlotting, WesternFluorescent Antibody TechniqueImmunoprecipitationTranscription Factor TFIIIAAnimalsCell Cycle ProteinsHumansMembrane Transport ProteinsAntibodiesCell Cycle ProteinsMembrane Transport ProteinsOPTN protein, humanTranscription Factor TFIIIAantibody characterizationantibody validationimmunofluorescenceimmunoprecipitationoptineurinOPTNQ96CV9western blot

Identifiers

PMID41716354
PMCPMC12914170

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.