Evidence map›Paper›PMID 41716320›Full record

ArticleFrontiers in veterinary science2026

Development of a TaqMan-based triplex qPCR assay for the simultaneous detection of Muscovy reovirus, novel duck reovirus, and

Zhihanlin Gan, Gaofeng Cai, Haiqin Li, Zhanhong Zheng, Linjie Song, Yiyang Zhang, Ping Liu, Yiwen Chen, Zhixuan Shen, Xiaona Gao and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhihanlin Gan *Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Gaofeng Cai *Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Haiqin LiInstitute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, China.
Zhanhong ZhengJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Linjie SongJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Yiyang ZhangJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Ping LiuJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Yiwen ChenJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Zhixuan ShenJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Xiaona GaoJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Xiaoquan GuoJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: In duck breeding production, the incidence of infection of Riemerella anatipestifer (RA), Muscovy duck reovirus (MDRV), and novel duck reovirus (NDRV) is gradually increasing. Because the clinical symptoms caused by these three pathogens are relatively similar, it is quite difficult to distinguish and diagnose. Methods: This study aims to establish a TaqMan-based triplex qPCR detection assay that can detect the above three pathogens at the same time. Specifically designed, optimized and verified specific primers and probes, targeting the dtxr gene of RA and the σB protein coding gene of MDRV and NDRV, respectively. Results: The results show that the assay has good specificity, sensitivity and stability. The linear detection range of the three pathogens is 102~109 copies/μL and the minimum detection limit can reach 101copies. In this study, 99 clinical on-site samples were tested, and positive samples were detected. In addition, through the analysis of the drug-resistant gene spectrum of the RA isolate, it was found that it carries aminoglycosides (RanA, RanB, aadS), macrocyclic esters [EreD, Erm(35), ErmF], chloramphenicol (floR) and tetracyclines [Tet(X3), Tet(X6), TetX] drug resistance genes of antibacterial drugs. Discussion: In summary, the TaqMan-based triplex qPCR detection assay established in this study can be used as a fast, sensitive and reliable technical means for large-scale monitoring of RA, MDRV and NDRV infection; at the same time, the analysis of the supporting drug-resistant gene detection assay can provide reference for the rational use of antibacterial drugs in duck breeding production. Test the basis, and then help the breeding link to take scientific and effective prevention and control intervention measures in a timely manner.

Indexed as

Muscovy duck reovirusnovel duck reovirusRiemerella anatifestiferTaqMantriplex qPCR

Identifiers

PMID41716320
PMCPMC12913071

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