Evidence map›Paper›PMID 41716256›Full record

ArticleFrontiers in genetics2026

Maternal nutrition alters mRNA isoform expression, usage, and splicing dynamics in skeletal muscle of beef cattle offspring.

Guilherme Henrique Gebim Polizel, Ángela Cánovas, Germán D Ramírez-Zamudio, Aline Silva Mello Cesar, Wellison J S Diniz, José Bento Sterman Ferraz, Heidge Fukumasu, Luiz F Brito, Felipe Eguti de Carvalho, Miguel Santana

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In one paragraph

Article in Frontiers in genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Guilherme Henrique Gebim PolizelDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Ángela CánovasCentre for Genetic Improvement of Livestock (CGIL), Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada.
Germán D Ramírez-ZamudioDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Aline Silva Mello CesarDepartment of Food Science and Technology, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, Brazil.
Wellison J S DinizDepartment of Animal Sciences, College of Agriculture, Auburn University, Auburn, AL, United States.
José Bento Sterman FerrazDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Heidge FukumasuDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Luiz F BritoDepartment of Animal Sciences, Purdue University, West Lafayette, IN, United States.
Felipe Eguti de CarvalhoDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Miguel SantanaDepartment of Animal Science, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Maternal nutrition during gestation plays a critical role in fetal muscle development and long-term metabolic programming; however, its persistent molecular effects on offspring skeletal muscle remain unclear. Therefore, the main objective of this study was to investigate the influence of prenatal nutrition on long-term differential gene expression (DGE), differential mRNA transcript expression (DTE), and differential transcript usage (DTU) in skeletal muscle of beef cattle. Methods: A total of 126 pregnant Nellore cows were assigned to three dietary treatments: mineral supplementation only (NP), protein-energy supplementation during late gestation (PP), and protein-energy supplementation throughout gestation (FP). At 676 ± 28 days of age, muscle samples were collected from offspring for RNA sequencing. The DGE and DTE analyses were performed using the edgeR package, while DTU was evaluated with the IsoformSwitchAnalyzeR package. Over-representation analysis was conducted using g:Profiler. Results: A total of 27,412 genes and 111,185 transcripts, including novel loci and isoforms were identified. Gene-level differences were modest (16 genes), whereas transcript-level analyses revealed stronger effects, with a higher number of significant expression and usage changes across conditions. The FP × NP comparison exhibited the greatest impact on gene expression, with 14 DTEs and 87 DTUs, compared with 12 and 30 in PP × NP, and 10 and three in FP × PP, respectively. Isoform switching was observed in key genes including Conclusion: Overall, prenatal nutrition, particularly protein-energy supplementation throughout gestation in comparison to mineral supplementation, modulates offspring muscle mainly through transcript usage and splicing, suggesting long-term adaptive mechanisms beyond gene-level regulation.

Indexed as

amino acid transportfetal programminggene and transcript expressionregulatory mechanismsSLC gene familysplicing events

Identifiers

PMID41716256
PMCPMC12916067

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