ArticleiScience notes2024
SINGLE MOLECULE 3D STRUCTURES DETERMINED BY INDIVIDUAL-PARTICLE ELECTRON TOMOGRAPHY.
Article in iScience notes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
Macromolecules, such as proteins and nucleic acids, play essential roles in cellular functions through dynamic structural changes. Understanding these functions requires detailed characterization of structural dynamics. While techniques like X-ray crystallography and cryo-EM resolve high-resolution static structures, they struggle to capture low-resolution, flexible structures and the full distribution of conformations during chemical reactions. These limitations arise from averaging processes that enhance signal-to-noise ratio (SNR) but exclude flexible regions, distort resolution, and miss rare high-energy states. To address this, we developed individual-particle electron tomography (IPET), a method for determining 3D structures of single particles at low-to-intermediate resolution (up to 2 nm) without averaging. IPET reconstructs a detailed 3D density map by capturing images at multiple tilt angles, facilitating flexible model fitting and revealing unique particle structures. This method reveals unbiased structural distributions, enhancing the study of molecular dynamics, phase transitions, and structural alterations during chemical reactions and self-folding.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.