Evidence map›Paper›PMID 41716182›Full record

ArticleVeterinary world2025

Development and validation of a regionally adapted sandwich enzyme-linked immunosorbent assay targeting recombinant p60 antigen for rapid detection of

Guldarigash Kaukabayeva, Aigerim Turgimbayeva, Zhanar Akhmetkarimova, Sholpan Mukhlis, Gulkhan Unysheva, Sailau Abeldenov, Pavel Shevchenko, Albina Gabitova, Raushan Rychshanova, Aralbek Rsaliyev and 2 more

Abstract read
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Article in Veterinary world, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Guldarigash KaukabayevaStem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.
Aigerim TurgimbayevaStem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.
Zhanar AkhmetkarimovaStem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.
Sholpan MukhlisStem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.
Gulkhan UnyshevaStem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.
Sailau AbeldenovStem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.
Pavel ShevchenkoResearch Institute of Applied Biotechnology, Baitursynov Kostanay University, Mayakovskiy Street 99/1, Kostanay, 110000, Kazakhstan.
Albina GabitovaResearch Institute of Applied Biotechnology, Baitursynov Kostanay University, Mayakovskiy Street 99/1, Kostanay, 110000, Kazakhstan.
Raushan RychshanovaResearch Institute of Applied Biotechnology, Baitursynov Kostanay University, Mayakovskiy Street 99/1, Kostanay, 110000, Kazakhstan.
Aralbek RsaliyevStem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.
Yergali AbduraimovStem Cell Laboratory, National Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.
Saule EskendirovaNational Center for Biotechnology, 13/5 Korgalzhyn Highway, Astana, 010000, Kazakhstan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Materials and Methods: The Results: The recombinant p60 antigen (50.3 kDa) was successfully expressed and purified at 5.9 mg/L yield. Among seven stable hybridoma clones, mAb 1H8 exhibited the highest affinity (Ka = 2.5 × 10 Conclusion: The developed sandwich ELISA provides a sensitive, specific, rapid, and regionally tailored diagnostic platform for detecting pathogenic

Indexed as

foodborne pathogensfood safety surveillancehybridoma technologyimmunodiagnosticsListeria monocytogenesmonoclonal antibodiesOne Healthp60 antigenpolyclonal antibodiesrecombinant proteinsandwich enzyme-linked immunosorbent assay

Identifiers

PMID41716182
PMCPMC12913694

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.