Evidence map›Paper›PMID 41716157›Full record

ArticleVeterinary world2025

Molecular characterization and high prevalence of

Afaque H Syed, Mpinda Edoaurd Tshipamba, Ngoma Lubanza, Baitsholetsi G Mokolopi, Jean Marie Dibungi Luseba, Mulunda Mwanza

Abstract read
In one paragraph

Article in Veterinary world, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Afaque H SyedDepartment of Animal Health, School of Agriculture, Faculty of Natural and Agricultural Sciences, Mafikeng Campus, North-West University, Private Bag X2046, Mmabatho, 2735, South Africa.
Mpinda Edoaurd TshipambaCollege of Agriculture and Environmental Sciences, Department of Agriculture and Animal Health, University of South Africa, Florida Science Campus, Johannesburg, South Africa.
Ngoma LubanzaDepartment of Animal Health, School of Agriculture, Faculty of Natural and Agricultural Sciences, Mafikeng Campus, North-West University, Private Bag X2046, Mmabatho, 2735, South Africa.
Baitsholetsi G MokolopiCollege of Agriculture and Environmental Sciences, Department of Agriculture and Animal Health, University of South Africa, Florida Science Campus, Johannesburg, South Africa.
Jean Marie Dibungi LusebaUniversity of Kinshasa, Faculty of Veterinary Medicine, Democratic Republic of Congo.
Mulunda MwanzaDepartment of Animal Health, School of Agriculture, Faculty of Natural and Agricultural Sciences, Mafikeng Campus, North-West University, Private Bag X2046, Mmabatho, 2735, South Africa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Bovine trichomonosis, caused by Materials and Methods: A total of 239 sheath wash samples were collected between June 2018 and October 2020. Of these, 51 culture-positive trichomonad isolates were selected for molecular analysis. Microscopy and modified Giemsa staining were used to characterize protozoal morphology. DNA was extracted and subjected to RT-PCR with 5' TaqMan™ probes, as well as conventional PCR targeting the 5.8S rRNA/Internal Transcribed Spacer (ITS) regions. PCR amplicons were sequenced, and phylogenetic trees were constructed using MEGA (maximum-likelihood, 1,000 bootstrap replicates). Statistical comparisons between diagnostic methods were performed using Chi-square and Cochran's Q test. Results: RT-PCR detected Conclusion: This study confirms a high prevalence of

Indexed as

bovine trichomonosisconventional PCRphylogenyRT-PCRSouth AfricaTritrichomonas foetus

Identifiers

PMID41716157
PMCPMC12913826

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.