ArticleVeterinary world2025
First molecular insight into
Article in Veterinary world, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and Aim: The Materials and Methods: Blood samples from 50 Edilbay sheep reared at a breeding farm in Kazakhstan were subjected to DNA extraction and DRB1 exon 2 amplification using validated primers. Polymerase chain reaction products were purified and sequenced using Sanger sequencing. Allele identification was performed through pairwise sequence alignment in SnapGene and reference comparison with the Immuno Polymorphism Database of Major Histocompatibility Complex (IPD-MHC) database. Ambiguous chromatograms and overlapping nucleotide peaks were assessed for potential novel allelic patterns. Genetic diversity indices (Ho, He, Ne, and Shannon's H') were calculated. Results: A total of 25 known DRB1 alleles were identified in the Edilbay sheep population. Genetic diversity parameters demonstrated extremely high immunogenetic variation, with observed heterozygosity (Ho) of 0.94, expected heterozygosity (He) of 0.90, an effective number of alleles (Ne) of 16.7, and Shannon's index (H') of 3. Several chromatograms showed overlapping peaks or substitution patterns inconsistent with known alleles, including variations at positions 243-244 and multiple additional polymorphic sites. These patterns indicate the presence of putative novel alleles that could not be unambiguously assigned by direct Sanger sequencing. Approximately 20% of samples contained undocumented variants or low-quality chromatograms requiring further resolution. Conclusion: This study presents the first comprehensive molecular characterization of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.