Evidence map›Paper›PMID 41715233›Full record

ArticleMicrobiome2026

Comparative analysis of rumen metagenomes with dietary supplementation of 3-nitrooxypropanol revealed divergent modes of action in hydrogen metabolism and reductant pathways between beef and dairy cattle.

Youyoung Choi, Mi Zhou, Masahito Oba, Atmir Romero-Pérez, Karen A Beauchemin, Stephane Duval, Maik Kindermann, Le Luo Guan

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Youyoung ChoiFaculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
Mi ZhouFaculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
Masahito ObaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
Atmir Romero-PérezDepartment of Animal Nutrition and Biochemistry, Faculty of Veterinary Medicine and Animal Science, National Autonomous University of Mexico, Mexico City, 04510, Mexico.
Karen A BeaucheminAgriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada.
Stephane DuvalDSM Nutritional Products, Animal Nutrition and Health, Wurmisweg 576, Kaiseraugst, 4303, Switzerland.
Maik KindermannDSM Nutritional Products, Animal Nutrition and Health, Wurmisweg 576, Kaiseraugst, 4303, Switzerland.
Le Luo GuanFaculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada. leluo.guan@ubc.ca.

Funding

Beef Cattle Research Council Cluster FDE.18.21CFoundation for Food & Agriculture Research Greener Cattle Initiative Award ID 22-000373Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery and NSERC Alliance program ALLRP 588541-23
6 · The paper itself

Abstract

backgroundThe compound 3-nitrooxypropanol (3-NOP), an inhibitor of methyl-coenzyme M reductase (MCR), reduces enteric methane production in both beef and dairy cattle. Although the proposed mechanisms of 3-NOP involve on inhibiting the activity of MCR in vivo, it is unknown how this process could affect rumen microbiome as a whole and if it differs between beef and dairy cattle. This study conducted a comparative analysis of the rumen microbiome and its functional shifts in four different cattle studies (two beef and two dairy cattle studies) that evaluated 3-NOP supplementation using metataxonomics and metagenomics.

resultsComparative analysis of 281 rumen metataxonomic datasets (143 beef and 138 dairy cattle) revealed that dietary supplementation with 3-NOP affected rumen bacteria and methanogens. Further, comparative analysis of 54 metagenomic datasets (24 beef and 30 dairy cattle) revealed that 3-NOP inhibited mcrA, decreased the abundances of Methanobrevibacter gottschalkii and the protozoal species Isotricha prostoma, while increased the abundances of Methanobrevibacter ruminantium and Methanosphaera sp., Prevotella sp. was a significant bacterial taxon in both beef and dairy cattle, contributing to various pathways such as propionate and butyrate production. Its increased abundance after 3-NOP supplementation may also be linked to the decrease in Isotricha prostoma. Hydrogenotrophic methanogenesis decreased after 3-NOP supplementation with the abundance of genes involved in methylenetetrahydromethanopterin dehydrogenase decreased in beef cattle, while that of 4Fe-4S ferredoxin gene decreased in dairy cattle. The abundance of protozoal Polyplastron multivesiculatum increased after long-term 3-NOP supplementation in beef cattle, potentially due to changes in hydrogen (H

Indexed as

CattleGastrointestinal MicrobiomeMethanePropanolsRumenAnimalsDietary SupplementsFatty Acids, VolatileHydrogenMetabolic Networks and PathwaysMetagenome3-nitrooxypropanolFatty Acids, VolatileHydrogenMethanePropanols3-NitrooxypropanolBeef cattleComparative analysisDairy cattleMetagenomicsMetataxonomics

Identifiers

PMID41715233
PMCPMC12918512

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.