Evidence map›Paper›PMID 41714471›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2026

Nucleic acid detection based on single-cluster analysis of cross-linking aggregates of DNA-modified gold nanoparticles using a dark-field microscope.

Yuki Tanaka, Nanami Fukuzumi, Gen Hirao, Atsushi Ogawa, Tsuyoshi Asahi, Mizuo Maeda, Tamotsu Zako

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Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Yuki TanakaDepartment of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan.
Nanami FukuzumiDepartment of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan.
Gen HiraoDepartment of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan.
Atsushi OgawaProteo-Science Center, PIAS, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan.
Tsuyoshi AsahiDepartment of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan.
Mizuo MaedaRIKEN Pioneering Research Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Tamotsu ZakoDepartment of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan. zako.tamotsu.us@ehime-u.ac.jp.ORCID http://orcid.org/0000-0001-7106-5766

Funding

Ehime University Research Unit for Advanced Nano-BioanalysisJapan Society for the Promotion of Science 19H92527Japan Society for the Promotion of Science 22K19114Japan Society for the Promotion of Science 23K26471
6 · The paper itself

Abstract

Gold nanoparticles (AuNPs) have been utilised as colorimetric biosensors because of their optical properties. Single-stranded DNA (ssDNA)-immobilised AuNPs (ssDNA-AuNPs) are widely used for nucleic acid detection. AuNPs strongly scatter light, allowing individual particles to be observed using dark-field microscope (DFM). In this study, we performed target ssDNA detection using the cross-linked aggregation of ssDNA-AuNPs via DFM. The limit of detection (LOD) was determined by evaluating the aggregation ratio of the ssDNA-AuNPs estimated at the single-cluster level using DFM images. The LOD for the target ssDNA detection based on the intensity of each spot in the DFM image was 15 pM. To the best of our knowledge, this study is the first to perform a single-cluster analysis based on the red/gree/blue (RGB) values of individual spots in DFM images, and the LOD was determined to be 13 pM. These LOD values are approximately 50 times lower than those obtained using the colorimetric method. Our results indicate that single-cluster analysis based on the intensity or colour of each spot in DFM images enables sensitive detection of target molecules.

Indexed as

DNA, Single-StrandedGoldMetal NanoparticlesMicroscopyCluster AnalysisLimit of DetectionDNA, Single-StrandedGoldCrosslinking aggregationDark-field microscopeDNA detectionGold nanoparticlesSensitivity

Identifiers

PMID41714471

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