Evidence map›Paper›PMID 41714284›Full record

ArticleAlcohol, clinical & experimental research2026

Chronic intermittent ethanol vapor exposure alters local c-Fos and functional connectivity patterns evoked by alcohol cues.

Ankit Sood, Filip Hanak, Antony Joseph, M J Carpio, Runbo Gao, Adem Selimovic, Seth D C Weir, Fleur Uittenbogaard, Diana Augustin, Jocelyn M Richard

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Alcohol, clinical & experimental research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ankit SoodDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.ORCID https://orcid.org/0000-0002-5873-5785
Filip HanakDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
Antony JosephDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
M J CarpioDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
Runbo GaoDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
Adem SelimovicDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
Seth D C WeirDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
Fleur UittenbogaardDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
Diana AugustinDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
Jocelyn M RichardDepartment of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.ORCID https://orcid.org/0000-0001-5750-0418

Funding

Glutamatergic basal forebrain neurons in aversion-resistant drinkingR01AA028770 · NIAAA · UNIVERSITY OF MINNESOTA · PI RICHARD, JOCELYN M · 2021 to 2025
$2.2M
Ventral pallidal circuitry in alcohol seeking and reinstatement by stressR00AA025384 · NIAAA · UNIVERSITY OF MINNESOTA · PI RICHARD, JOCELYN M · 2018 to 2020
$747k
NIAAA NIH HHS R00 AA025384NIAAA NIH HHS R00AA025384NIAAA NIH HHS R01 AA028770NIAAA NIH HHS R01AA028770
6 · The paper itself

Abstract

backgroundChronic intermittent ethanol (CIE) vapor inhalation is a widely used model for studying alcohol dependence. Additionally, cues paired with ethanol can drive and invigorate seeking behavior. However, the impact of ethanol-paired cues on neural activation following a history of CIE vapor exposure remains poorly understood. Here, we examined the impact of ethanol-paired cues in rats with or without a history of CIE vapor exposure on neuronal activation in the medial prefrontal cortex (mPFC), nucleus accumbens (NAc), and the hippocampus (HPC) using c-Fos as a marker.

methodsMale and female Long-Evans rats with a history of air or CIE vapor exposure were exposed to previously learned alcohol-associated cues under extinction conditions. Rats were perfused either immediately following cue exposure or with a delay to allow the development of c-Fos protein. c-Fos expression levels were analyzed in the mPFC, NAc, and HPC.

resultsCIE vapor exposure led to a region-specific effect on cue-evoked c-Fos levels, with enhanced cue-evoked c-Fos levels observed in the prelimbic mPFC of CIE rats. Correlation analysis of c-Fos expression across brain regions revealed that CIE vapor exposure also led to altered functional connectivity patterns.

conclusionsOur results suggest that CIE vapor exposure affects cue-evoked c-Fos expression in the prelimbic mPFC as well as influencing functional connectivity across regions.

Indexed as

CuesEthanolProto-Oncogene Proteins c-fosAdministration, InhalationAnimalsFemaleHippocampusMaleNucleus AccumbensPrefrontal CortexRatsRats, Long-EvansEthanolProto-Oncogene Proteins c-fosalcohol cuesethanol vaporhippocampusnucleus accumbensprefrontal cortex

Identifiers

PMID41714284
PMCPMC12920271

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.