Evidence map›Paper›PMID 41709861›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

miR-185-5p Derived From hUC-MSC Exosomes via Suspension Culture Under Hypoxic Conditions Promotes Scarless Wound Healing in Mice by Precisely Regulating Collagen I/III Regeneration.

Lihua Yang, Yiqun Sun, Qinbiao Yan, Ali Mohsin, Kamran Ashraf, Senyi Gong, Weifeng Li, Touseef Ur Rehman, Yuwen Hu, Jinzhao He and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Lihua YangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Yiqun SunDepartment of Burn and Plastic Surgery, Hainan Western Central Hospital, Danzhou, Hainan, China.
Qinbiao YanState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Ali MohsinState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Kamran AshrafState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Senyi GongState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Weifeng LiState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Touseef Ur RehmanState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Yuwen HuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Jinzhao HeState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Yu LiuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Meijun DingDepartment of Burn and Plastic Surgery, Affiliated Hospital of West Anhui Health Vocational College, Luan, Anhui, China.
Lin QiDepartment of Radiology, Huadong Hospital, Fudan University, Shanghai, Shanghai, China.
Meijin GuoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Shanghai, China.
Ke XueDepartment of Burn and Plastic Surgery, Hainan Western Central Hospital, Danzhou, Hainan, China.ORCID https://orcid.org/0000-0001-5347-2712

Funding

Hainan Provincial Postdoctoral Science Foundation 822CXTD537Hainan Provincial Postdoctoral Science Foundation 823MS157National Natural Science Foundation of China 82272290Strategic Priority Research Program of the Chinese Academy of Sciences XDA16030702
6 · The paper itself

Abstract

Hypertrophic scars have long posed a significant challenge in wound healing, primarily because of an imbalance between Type I (COL-I) and Type III (COL-III) collagen caused by excessive fibroblast activation. Existing treatments cannot directly regulate collagen formation to intervene in scar hyperplasia. This study is the first to elucidate the key mechanism through which human umbilical cord-derived mesenchymal stem cell (hUC-MSCs) exosomes expressing miR-185-5p achieve scarless healing through remodeling of the collagen-type ratio. In this method, a hypoxic three-dimensional (3D-HO) suspension culture system that more closely mimics the in vivo microenvironment was established, and functional exosomes with enhanced physiological activity and higher yields were harvested. In vivo experiments demonstrated that 3D-HO exosomes significantly improved the COL-III/COL-I regeneration ratio during mouse wound healing. Mechanistic studies revealed that the RhoA/YAP signaling axis plays a key regulatory role in the collagen regeneration ratio. Further molecular analysis revealed enrichment of miR-185-5p in 3D-HO exosomes, which directly target RhoA to regulate fibroblasts. Both in vivo and in vitro functional interventions confirmed miR-185-5p in maintaining collagen type balance. In summary, this study demonstrates that microenvironment-enhanced hUC-MSCs exosomes remodel the composition of different types of collagen via the miR-185-5p-RhoA/YAP signaling axis, thereby driving scarless healing.

Indexed as

Collagen Type ICollagen Type IIIExosomesMesenchymal Stem CellsMicroRNAsWound HealingAnimalsHumansMiceCollagen Type ICollagen Type IIIMicroRNAsMIRN185 microRNA, humancollagen ratiohypoxic exosomesmiR‐185‐5pscarless healingthree‐dimensional suspension culture

Identifiers

PMID41709861
PMCPMC13042918

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.