Evidence map›Paper›PMID 41709464›Full record

ArticleCell reports methods2026

Non-invasive ovulation tracking enables genetic engineering in wild rodents.

Joanna Buchthal, Emma J Chory, Zachary Hill, Christy Dennison, Boqiang Tu, Rick P Wierenga, Çağrı Çevrim, Stefan Golas, Gabriel Meier, Tammy C T Lan and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Joanna BuchthalMedia Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Mice Against Ticks, Lincoln, MA 01773, USA. Electronic address: buchthal@mit.edu.
Emma J ChoryMedia Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biomedical Engineering, Duke University, Durham, NC 27705, USA.
Zachary HillMedia Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Christy DennisonMedia Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Boqiang TuMedia Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Rick P WierengaMedia Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Çağrı ÇevrimDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Stefan GolasMedia Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Gabriel MeierWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; RNA Biology and Molecular Physiology, Faculty for Biology, Bielefeld University, Bielefeld, Germany.
Tammy C T LanBroad Institute of Harvard and MIT, Cambridge, MA 02142, USA; Department of Molecular and Cellular Biology, Harvard University, Boston, MA, USA.
Hattie ChungYale University School of Medicine, New Haven, CT 06520, USA.
Magalie BoucherDivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Sam R TelfordDepartment of Infectious Disease and Global Health, Tufts University, Grafton, MA 01536, USA.
Kara L McKinleyDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute, Cambridge, MA 02138, USA.
Styliani MarkoulakiWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Rudolf JaenischWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Kevin M EsveltMedia Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: esvelt@mit.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many non-model rodent species are inaccessible to genetic engineering due to our limited understanding of their reproductive biology. Here, we present a low-cost, camera-based estrous-tracking technology that enables transgenesis in the white-footed mouse Peromyscus leucopus, a key reservoir for Lyme disease. We demonstrate the efficient generation of pregnant and pseudopregnant mice via timed ovulation, provide protocols for embryo generation, cultivation, microinjection, and transplantation as well as an accurate developmental timeline, and report the first engineered Peromyscus. The same technology successfully tracked conserved estrous-linked cycling behavior in other rodents, including hamsters. Finally, estrous tracking differentiated reproductively healthy, geriatric female Peromyscus from those with declining fertility based solely on their activity, providing a non-invasive method for studying reproductive senescence. Collectively, these tools represent a critical resource for engineering non-model rodents, advance the long-lived Peromyscus as a model organism, and will prove essential to heritably immunizing wild rodent populations against Lyme disease.

Indexed as

Genetic EngineeringOvulationPeromyscusAnimalsAnimals, WildCricetinaeFemaleLyme DiseaseMicePregnancyReproductionautomated activity monitoringCP: developmental biologyCP: geneticsCRISPR-Cas9 transgenesisestrous cycle trackingLyme disease reservoirmachine learning in cytologynon-invasive phenotypingnon-model rodentsPeromyscus leucopusreproductive senescencewhite-footed mouse

Identifiers

PMID41709464
PMCPMC12946743

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.