Evidence map›Paper›PMID 41709302›Full record

ArticleBMC veterinary research2026

An organoid-based evaluation of Bacillus amyloliquefaciens SC06 in alleviating oxidative damage via Wnt signaling.

Li Tang, Hao Wang, Qi Wang, Yang Wang, Xiang Li, Fei Wang, Qian Jin, Zihan Zeng, Aikun Fu, Xiaoliang Li and 1 more

Abstract read
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In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Li Tang *Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China.
Hao Wang *Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China.
Qi Wang *Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Yang WangCollege of Animal Sciences, Qingdao Agricultural University, Qingdao, 266000, China.
Xiang LiKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Fei WangKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Qian JinKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Zihan ZengKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Aikun FuKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Xiaoliang LiKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. xlli@zju.edu.cn.
Weifen LiKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. wfli@zju.edu.cn.

Funding

Shandong Provincial Natural Science Foundation No. ZR2020QC183the National Natural Science Foundation of China No.32072766the Natural Science Foundation of Zhejiang province No. LZ20C170002the "Pioneer" and "Leading" key R&D Program of Zhejiang Province No. 2023C02036，No.2023C02023
6 · The paper itself

Abstract

Bacillus amyloliquefaciens SC06 (BaSC06) has emerged as a promising probiotic for improving animal gut health and immuno-protection. However, the underlying molecular mechanisms remain incompletely understood. In this study, we employed porcine intestinal organoids as an ex vivo model and induced oxidative stress using diquat to systematically evaluate the protective effects of BaSC06. We evaluated antioxidant capacity, apoptosis-related markers, intestinal stem cells differentiation markers, and associated signaling pathways. Pretreatment with BaSC06 significantly alleviated oxidative damage by reducing intracellular reactive oxygen species levels and resultant apoptosis. Cellularly, BaSC06 promoted intestinal stem cells proliferation and favored differentiation toward Paneth cells while suppressing differentiation into other epithelial lineages. Mechanistically, these effects were mediated by the activation of the Wnt signaling pathway, and it was further confirmed by using a specific Wnt inhibitor. Overall, our findings uncover a probiotic-Wnt axis through which BaSC06 confers protection against oxidative stress, involving modulation of intestinal stem cells fate and enhancement of epithelial barrier function.

Indexed as

Bacillus amyloliquefaciensOrganoidsOxidative StressProbioticsWnt Signaling PathwayAnimalsApoptosisCell DifferentiationDiquatIntestinesReactive Oxygen SpeciesStem CellsSwineDiquatReactive Oxygen SpeciesBacillus amyloliquefaciensIntestinal stem cellsOxidative stress damagePorcine intestinal organoidWnt signaling pathway

Identifiers

PMID41709302
PMCPMC13020122

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.