ArticleJournal of applied toxicology : JAT2026
Single Intraperitoneal Busulfan Injection Induces Long-Term Reproductive Dysfunction and Reduces Male Offspring Ratio in BALB/c Mice.
Article in Journal of applied toxicology : JAT, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Single Intraperitoneal Busulfan Injection Induces Long-Term Reproductive Dysfunction and Reduces Male Offspring Ratio in BALB/c Mice.Journal of applied toxicology : JAT · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
This study aims to assess the long-term effects of busulfan on male reproductive function in BALB/c mice to provide insight into its toxicological effects. A total of 80 BALB/c mice were randomly assigned to a control group (n = 20) and three experimental groups (n = 20 each), which received a single intraperitoneal injection of busulfan at 15, 20, and 25 mg/kg, respectively. A subset of mice was assessed at multiple time points for spermatogenesis. Additionally, fertility experiments with both sexes served as a vital measure of fertility potential. Results revealed that relative testicular weight and semen quality in the experimental groups were significantly decreased compared with those in the control group (p < 0.05). Abnormal sperm morphology showed a significant increase (p < 0.05), with a notable correlation observed between abnormal sperm neck morphology and reduced sperm motility (p < 0.05). Fertility trials indicated that busulfan-treated males had a reduced ability to impregnate females, as evidenced by reductions in pregnancy rates and litter sizes. Remarkably, the male offspring ratio in the busulfan-treated group (7 out of 17) was significantly lower than that in the control group (21 out of 42). In conclusion, a single intraperitoneal injection of busulfan led to sustained fertility impairment in mice for at least 13 weeks, with potential transgenerational effects, including alterations in the sex ratio of male descendants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.