ArticlePoultry science2026
Dietary evaluation of Pleurotus ostreatus spent mushroom substrate on productivity, nutrient digestibility, egg quality, antioxidant status, and ovarian cytoprotective responses in laying hens.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study evaluated the dietary inclusion level effects of Pleurotus ostreatus spent mushroom substrate (SMS) on performance, nutrient digestibility, egg quality and oxidative stability in laying hens. Additionally, key ovarian homeostasis-related genes involved in detoxification (aryl hydrocarbon receptor-AhR), antioxidant (nuclear factor erythroid 2-related factor 2-Nrf2), and inflammatory response (nuclear factor kappa B-NF-κB) were assessed. A total of 196 Hy-Line Brown hens, 51-weeks of age, were assigned to 4 treatments with 7 replicates of 7 hens each, receiving isocaloric, isonitrogenous and isofibrous diets containing 0 (S0), 25 (S25), 50 (S50), or 100 (S100) g SMS/kg. Performance responses were determined weekly throughout the 7-week experimental period and are reported as overall. At the end of week 7, eggs were collected for quality and oxidative stability determination, and ovarian samples were obtained for qPCR analysis. Increasing SMS inclusion level, improved overall laying rate and egg mass, with optimal (P ≤ 0.05) performance notable in the S100 compared to S0, and enhanced ether extract digestibility (P < 0.01). Egg oxidative stability was significantly improved by SMS inclusion, as reflected by reduced yolk malondialdehyde and egg-white protein carbonyl levels in fresh and stored eggs (P ≤ 0.001), with the lowest values consistently observed in the S100 treatment. The nutrigenomic analyses revealed that dietary SMS inclusion beneficially modulated (P ≤ 0.05) the majority of ovary-expressed genes related to detoxification (AhR pathway; 3/4 genes), antioxidant defense (Nrf2 pathway; 5/9 genes), and inflammatory response (NF-κB pathway; 8/13 genes), with the most pronounced differences observed in the S100 treatment compared with the control treatment. Conclusively, dietary SMS inclusion at 100 g/kg enhanced layer performance, improved ether extract digestibility, and increased egg oxidative stability. In addition, nutrigenomic analyses revealed that the observed improvements in performance and egg quality were supported by beneficial modulation of key homeostasis-related genes relevant to ovarian function and health.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.