Evidence map›Paper›PMID 41706846›Full record

ArticleScience advances2026

3D reconstruction of human liver tissue at cellular resolution.

Wesley B Fabyan, Chelsea L Fortin, Dorice L Goune, Heidi L Kenerson, Susana P Simmonds Bohorquez, Jonathan T C Liu, Matthew M Yeh, Rotonya M Carr, Raymond S W Yeung, Kelly R Stevens

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. A 3D In Vitro Model of the Human Hepatobiliary Junction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Wesley B FabyanDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-1829-6511
Chelsea L FortinDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-6931-0862
Dorice L GouneDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0005-2031-3148
Heidi L KenersonDepartment of Surgery, University of Washington, Seattle, WA 98195, USA.
Susana P Simmonds BohorquezDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA.ORCID 0009-0001-3400-8479
Jonathan T C LiuDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0001-5650-3086
Matthew M YehDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA.
Rotonya M CarrDepartment of Medicine, Division of Gastroenterology, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0003-0235-6994
Raymond S W YeungDepartment of Surgery, University of Washington, Seattle, WA 98195, USA.
Kelly R StevensDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-4024-2990

Funding

Photoabsorbing bioinks for expanding 3D printed human liver in situR01DK128551 · NIDDK · UNIVERSITY OF WASHINGTON · PI STEVENS, KELLY R · 2021 to 2024
$3.0M
NIDDK NIH HHS R01 DK128551
6 · The paper itself

Abstract

The liver contains an intricate microstructure that is critical for proper liver function. Architectural disruption of this spatial structure is pathologic. Unfortunately, two-dimensional (2D) histopathology-the gold standard for pathological understanding of many liver diseases-can misrepresent or leave gaps in our understanding of complex 3D structural features. Here, we used immunostaining, tissue clearing, microscopy, and computational software to create 3D multilobular reconstructions of both nonfibrotic and cirrhotic human liver tissue. We found that spatial architecture in human cirrhotic liver samples with varying etiologies had sinusoid zonal dysregulation, reduction in glutamine synthetase-expressing pericentral hepatocytes, regression of central vein networks, disruption of hepatic arterial networks, and fragmentation of biliary networks, which together suggest a pro-portalization/decentralization phenotype in cirrhotic tissue. Further implementation of 3D pathological analyses may provide a deeper understanding of cirrhotic pathobiology and inspire treatments for liver disease.

Indexed as

Imaging, Three-DimensionalLiverLiver CirrhosisHepatocytesHumans

Identifiers

PMID41706846
PMCPMC12915621

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.