Evidence map›Paper›PMID 41706346›Full record

ArticleMycotoxin research2026

Aflatoxin B

Adey Melesse Yalew, Alemayehu Teressa Negawo, Tesfaye Alemu, Jean Hanson, Michael Blummel, Nebiyat Nigusie Woldeyohannis, Yilikal Assefa Teferi, Yonas Asmare, Prasad Kodukula V S V, Chris S Jones

Abstract read
In one paragraph

Article in Mycotoxin research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Adey Melesse YalewDepartment of Microbial, Cellular and Molecular Biology, Addis Ababa University (AAU), Addis Ababa, Ethiopia.
Alemayehu Teressa NegawoFeed and Forage Development, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia.
Tesfaye AlemuDepartment of Microbial, Cellular and Molecular Biology, Addis Ababa University (AAU), Addis Ababa, Ethiopia.
Jean HansonFeed and Forage Development, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia.
Michael BlummelFeed and Forage Development, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia.
Nebiyat Nigusie WoldeyohannisDepartment of Microbial, Cellular and Molecular Biology, Addis Ababa University (AAU), Addis Ababa, Ethiopia.
Yilikal Assefa TeferiFeed and Forage Development, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia.
Yonas AsmareFeed and Forage Development, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia.
Prasad Kodukula V S VFeed and Forage Development, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia.
Chris S JonesFeed and Forage Development, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia. C.S.Jones@cgiar.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study was conducted in Addis Ababa, surrounding peri-urban and urban feed markets to assess the prevalence of aflatoxin B1 (AFB1) and identify fungal diversity in livestock feeds. Two hundred and ninety samples of oil seed cakes and formulated feeds were collected from the randomly selected feed vendors. The samples were analysed for aflatoxin B1 using a Helica® Aflatoxin B1 (low matrix) Enzyme-linked immunoassay (ELISA) kit. To validate the ELISA results, a subset of randomly selected samples was further analysed using High-performance liquid chromatography-fluorescence detection (HPLC-FLD). The results indicated that 92% of poultry ration, 72% of dairy ration, 66% of linseed cake, 64% of noug seed cake, 61% of cotton seed cake, and 36% of soybean cake were contaminated with AFB1 exceeding the permissible level (20 µg/kg) in Ethiopia. To evaluate fungal diversity, genomic DNA was extracted, pooled by feed type, and sequenced. The findings revealed variations in the diversity indices of fungal phyla, families, genera and species. The Metabarcoding results revealed Ascomycota as the dominant phylum in all feeds, with Aspergillaceae, Nectriaceae, Botyosphaeriaceae, and Cladosporiaceae being the dominant families. Aspergillaceae accounts for 53%, 43%, 16%, 33%, 11%, and 28% of the total fungal contamination detected in cotton seed cake (S1), noug seed cake (S2), soybean cake (S3), linseed cake (S4), poultry ration (S5), and dairy ration (S6), respectively. The synergistic presence of multiple fungal species, some of which are known to be harmful, was identified in the feed samples. Among the species, the proportion of Aspergillus flavus found was 1.8%, 3.8%, 0%, 0.4%, 1.2%, and 1.2% in the feeds S1–S6, respectively. The findings highlight the critical need for enhanced feed management practices, improved education and training for feed handlers on feed safety, stricter regulatory measures, and more research on mycotoxins mitigation.

Indexed as

Aflatoxin B1Animal FeedFood ContaminationFungiSeedsAnimalsBiodiversityChromatography, High Pressure LiquidEnzyme-Linked Immunosorbent AssayEthiopiaLivestockAflatoxin B1Aspergillus flavusConcentrateFeed safetyFungal contaminationMycotoxin

Identifiers

PMID41706346
PMCPMC12917036

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.