ArticleCurrent microbiology2026
Eco-friendly Suppression of Xanthomonas axonopodis pv. punicae: the Role of Lactobacillus helveticus PMP-6 in Pomegranate Disease Management.
Article in Current microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The present study evaluates the antibacterial efficacy of a bacteriocin synthesized by Lactobacillus helveticus PMP-6 (GenBank accession no. MT444488) against Xanthomonas axonopodis pv. punicae (Xpp-1), the etiological agent of bacterial blight in Punica granatum L. (Pomegranate). The bacteriocin underwent purification, leading to a substantial increase in its specific activity from 233.849 AUmg-1 to 3615.81 AUmg-1, confirming successful enrichment of the bioactive peptide. Antibacterial assays demonstrated inhibitory effects under optimal conditions of 40 °C and pH 7.0, with a Minimum Inhibitory Concentration of 300 µgmL-1 and a Minimum Bactericidal Concentration of 400 µgmL-1. The antibacterial effect diminished in the presence of proteolytic enzymes, suggesting its proteinaceous nature. SDS-PAGE profiling of purified bacteriocin revealed a molecular weight of approximately 17 kDa. Furthermore, a Detached Leaf Assay validated it's in planta effectiveness, as no visible disease symptoms were observed in treated pomegranate leaves up to 96 h. of post-inoculation. Collectively, these results underscore the potential of L. helveticus PMP-6-derived bacteriocin as a environmentally sustainable biocontrol agent for managing bacterial blight disease of pomegranate, providing a viable alternative to conventional chemical pesticides.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.