ArticleReproduction & fertility2026
Effect of freezing and cryostorage on follicular fluid vitamin D levels.
Article in Reproduction & fertility, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
5 authors.
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Abstract
Lay summary: In the studies assessing the relationship between vitamin D levels and pregnancy outcomes in in vitro fertilization, doctors sometimes collected and froze the follicular fluid (fluid around eggs in a woman's ovaries) so they can test it later for research. This study aimed to find out if freezing and storage changes the level of vitamin D in that fluid, which could affect how reliable the tests are. We collected follicular fluid of 20 women going through in vitro fertilization. The samples were split into portions and were frozen at two different temperatures: -20 and -80°C. Vitamin D levels were analysed at baseline (before freezing), after 2 weeks and again after 7 months. The results showed that freezing and storage did not make a noticeable difference in vitamin D levels. That means storing the fluid this way for up to 7 months is an acceptable method for research.
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