ArticleNucleic acids research2026
A rapid test for protein-DNA interactions.
Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- CaHoT-GRN: context-aware high-order topology learning for robust single-cell gene regulatory network inference.Briefings in bioinformatics · 2026Article
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4 authors.
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Abstract
The characterization of protein-DNA interactions underpinning fundamental biological processes requires methods that are laborious and time-consuming. Here, we introduce a rapid and instrument-free assay leveraging on GFP-tagged proteins and the lateral flow assay principle to examine protein-DNA interactions. The rapid protein-DNA interaction test (R-PNAI-T) detects complexes using a dipstick in ∼15 min. Validation of the R-PNAI-T with bacterial proteins involved in replication and transcription demonstrated its applicability for diverse protein-DNA interactions, achieving a remarkable detection sensitivity of ∼0.7 fmol with the Escherichia coli Tus protein. Analysis of these protein interactions with their specific target DNA sequences highlighted the capability of the R-PNAI-T to discern subtle differences in affinity, providing valuable comparative data. The R-PNAI-T is robust, retaining functionality in human serum and bacterial lysates. The assay showed tolerance towards biotin contaminants, expanding its use for quality control in protein purification processes and tracking complexes in biological samples. To demonstrate versatility, we applied the R-PNAI-T with polymerase chain reaction-amplified DNA to probe the putative origin of replication in Burkholderia pseudomallei, confirming a functional interaction between the DnaA initiator protein and the DnaA box in this bacterium. Overall, the R-PNAI-T is versatile, cost-effective, and user-friendly, offering broad applications in biological research and biotechnology.
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