Evidence map›Paper›PMID 41704321›Full record

ArticleRSC chemical biology2026

Optical control of Cas9 activity through visible-light cleavable crRNAs.

Vanessa Hanff, Stepan Jerabek, Kim A Langer, Robin Klimek, Janik Kaufmann, Jimin Kim, Dieter Egli, Alexander Heckel

Abstract read
In one paragraph

Article in RSC chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Vanessa HanffInstitute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt Max-von-Laue-Str. 9 Frankfurt am Main 60438 Germany heckel@uni-frankfurt.de.
Stepan JerabekDepartment of Pediatrics and Naomi Berrie Diabetes Center, Columbia University New York NY 10032 USA.
Kim A LangerInstitute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt Max-von-Laue-Str. 9 Frankfurt am Main 60438 Germany heckel@uni-frankfurt.de.
Robin KlimekInstitute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt Max-von-Laue-Str. 9 Frankfurt am Main 60438 Germany heckel@uni-frankfurt.de.
Janik KaufmannInstitute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt Max-von-Laue-Str. 9 Frankfurt am Main 60438 Germany heckel@uni-frankfurt.de.
Jimin KimColumbia University Stem Cell Initiative New York NY 10032 USA.
Dieter EgliDepartment of Pediatrics and Naomi Berrie Diabetes Center, Columbia University New York NY 10032 USA.
Alexander HeckelInstitute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt Max-von-Laue-Str. 9 Frankfurt am Main 60438 Germany heckel@uni-frankfurt.de.ORCID https://orcid.org/0000-0003-3541-4548

Funding

Medical Scientist Training Program Training GrantT32GM145449 · NIGMS · DUKE UNIVERSITY · PI Christopher D Kontos · 2022 to 2026
$6.6M
NIGMS NIH HHS T32 GM145449
6 · The paper itself

Abstract

In this work, we present several visible-light cleavable crRNAs targeting two different genes for regulating Cas9 activity. The crRNAs contain a total of three different photocleavable linker (PL) which induce a bond break when irradiated with a suitable wavelength. Cleavage of the crRNAs efficiently inhibits Cas9 activity, as demonstrated by the

Identifiers

PMID41704321
PMCPMC12908655

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.