Evidence map›Paper›PMID 41704216›Full record

ArticleRNA biology2026

Cas10 residues lining the target RNA binding channel regulate interference by distinguishing cognate target RNA from mismatched targets.

Sarah A Khweis, Mason A Blackburn, Calvin C Perdigao, Megan O Pierce, Colby R Lewis, Jack A Dunkle

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sarah A KhweisDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.ORCID 0000-0002-7727-7265
Mason A BlackburnDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.
Calvin C PerdigaoDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.ORCID 0009-0001-4996-7496
Megan O PierceDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.
Colby R LewisDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.
Jack A DunkleDepartment of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL, USA.ORCID 0000-0001-6252-0587

Funding

Determining the specificity and regulation of Type III CRISPR-Cas interferenceR35GM142966 · NIGMS · UNIVERSITY OF ALABAMA IN TUSCALOOSA · PI DUNKLE, JACK ALBERT · 2021 to 2025
$1.8M
NIGMS NIH HHS R35 GM142966
6 · The paper itself

Abstract

Type III CRISPR systems are defined by the presence of the Cas10 protein and are among the most abundant CRISPR systems in nature. Cas10 forms a complex with crRNA and several Cas proteins that surveils prokaryotic cells for foreign RNA molecules and when they are detected it activates a cascade of interference activities. The synthesis of the cyclic oligoadenylate signalling molecule by Cas10 is a key aspect of the interference cascade. Despite structures of the Cas10 complex bound to target RNAs, the molecular mechanism by which Cas10 senses the bound state to licence interference is lacking. We identified five residues in

Indexed as

Bacterial ProteinsCRISPR-Associated ProteinsCRISPR-Cas SystemsRNA, BacterialRNA-Binding ProteinsBinding SitesProtein BindingBacterial ProteinsCRISPR-Associated ProteinsRNA, BacterialRNA-Binding ProteinsCas10CRISPR-Cascyclic oligoadenylateinterferencetype III

Identifiers

PMID41704216
PMCPMC12934148

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.