Evidence map›Paper›PMID 41703933›Full record

ArticlePlant communications2026

Telomere-to-telomere genome assembly and comprehensive mutation library facilitate bitter gourd breeding and functional genomics research.

Yilin Zhang, Xiaoyi Wang, Yan Wang, Yu Niu, Xu Han, Jingsheng Tan, Xinyu Ma, Qingzheng Zhang, Jie Liu, Zhijie Zhang and 8 more

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Article in Plant communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

18 authors.

Yilin ZhangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang 261000, China; Laboratory of Plant and Environment Interaction Study, Tsientang Institute for Advanced Study, Hangzhou 310024, China; State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, Beijing 100871, China; College of Life Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: yilinzhang@stu.pku.edu.cn.
Xiaoyi WangTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, 572024, Hainan, China.
Yan WangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang 261000, China.
Yu NiuTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou 571101, China; Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, China.
Xu HanTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou 571101, China; Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, China.
Jingsheng TanNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang 261000, China.
Xinyu MaNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang 261000, China.
Qingzheng ZhangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang 261000, China.
Jie LiuTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; College of Tropical Agricultural and Forestry, Hainan University, Haikou 570228, Hainan, China.
Zhijie ZhangTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; College of Tropical Agricultural and Forestry, Hainan University, Haikou 570228, Hainan, China.
Wenying GaoTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; College of Tropical Agricultural and Forestry, Hainan University, Haikou 570228, Hainan, China.
Wenjie LiangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang 261000, China.
Luyao LiTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; College of Horticulture & Forestry Sciences of Huazhong Agricultural University, Wuhan 430070, China.
ZhaoHua LiuTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou 571101, China; Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, China.
Xingping ZhangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang 261000, China. Electronic address: xingping.zhang@pku-iaas.edu.cn.
Hang HeNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang 261000, China; State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, Beijing 100871, China. Electronic address: hehang@pku.edu.cn.
Yan YangTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, 572024, Hainan, China; Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou 571101, China; Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, China. Electronic address: yanyang@catas.cn.
Renbo YuTropical Crops Genetic Resources Institute, State Key Laboratory of Tropical Crop Breeding, Chinese Academy of Tropical Agricultural Sciences, Haikou/Sanya, Hainan 571101/572024, China; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, 572024, Hainan, China; Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou 571101, China; Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, China. Electronic address: yurenbo612@163.com.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

Bitter gourd (Momordica charantia) is an economically important vegetable crop with notable medicinal properties, yet its genetic improvement has been hindered by limited genomic resources and insufficient functional genomics tools. Here, we present a comprehensive genomic and functional resource platform to address these limitations. Using PacBio HiFi and Oxford Nanopore ultra-long sequencing technologies combined with Hi-C, we generated a telomere-to-telomere (T2T), gap-free genome assembly of the elite bitter gourd cultivar Y52, comprising 11 chromosomes with a final assembly size of 298.0 Mb. This high-quality assembly enabled precise annotation of complex genomic regions, including centromeres characterized through chromatin immunoprecipitation sequencing. We further constructed a large-scale ethyl methanesulfonate (EMS)-induced mutant library consisting of 3223 M

Indexed as

Genome, PlantMomordica charantiaPlant BreedingTelomereGene LibraryGenomicsMutationcentromere evolutionEMS mutant libraryMutMappan-transcriptomicsQTL-seqT2T genome

Identifiers

PMID41703933
PMCPMC13084101

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.