ArticleJournal of cachexia, sarcopenia and muscle2026
Single-Cell RNAseq Identifies Heterogeneity in Myoblasts From Older Adults With Differences Related to Muscle Mass and Function.
Article in Journal of cachexia, sarcopenia and muscle, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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Who cites it
3 citing papers in PubMed.
- Review
- Sarcopenia and Skeletal Muscle Dysfunction in Liver Cirrhosis: Clinical Perspectives of Myostatin Inhibition.Biomedicines · 2026Review
- Single-Cell RNAseq Identifies Heterogeneity in Myoblasts From Older Adults With Differences Related to Muscle Mass and Function.Journal of cachexia, sarcopenia and muscle · 2026Article
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Authors and funding
12 authors.
Funding
Abstract
backgroundAgeing is associated with the loss of muscle mass and function, with consequences for metabolic health, frailty and independence in later life. The aim of this study was to investigate the transcriptional heterogeneity of human proliferating muscle satellite/stem cells (myoblasts) from older adults and how this heterogeneity may vary between healthy individuals and those with low muscle mass and function.
methodsSingle-cell transcriptomic analysis was carried out on proliferating myoblasts isolated from vastus lateralis biopsies from 132 participants (34 male, 98 female) aged 72-83 years from the Hertfordshire Sarcopenia Study extension. Uniform Manifold Approximation and Projection (UMAP) clustering was applied to identify clusters of myoblasts with distinct transcriptional profiles, Gene Ontology analysis was used to identify pathways enriched among the clusters, and pseudotime trajectory analysis was used to identify inferred cell lineages. Differential gene expression within cell clusters, together with the proportions of cells within each cluster and lineage, were assessed with respect to participant appendicular lean-mass index (ALMi), grip strength, and gait speed.
resultsThirteen distinct cell clusters based on the transcriptional heterogeneity of the myoblasts were identified. Clusters 0-6 contained the majority (94.6%) of cells. Marker genes were enriched for cytoplasmic translation (Cluster 0, false discovery rate [FDR] = 7.21 × 10
conclusionOur findings demonstrate considerable transcriptional heterogeneity in skeletal muscle myoblasts from older adults. This heterogeneity includes myoblasts from individuals with low muscle mass and strength progressing towards a fibrogenic or stressed state.
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