Evidence map›Paper›PMID 41703631›Full record

ArticleGenome biology2026

Non-disruptive in vitro monitoring of cellular states with cell-free DNA methylation.

Anja Hess, Alexander Kovacsovics, Fabian Bachinger, Ludovic Vallier, Helene Kretzmer, Alexander Meissner

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Anja HessMax Planck Institute for Molecular Genetics, Berlin, Germany.ORCID http://orcid.org/0000-0001-6634-9502
Alexander Kovacsovics *Max Planck Institute for Molecular Genetics, Berlin, Germany.
Fabian Bachinger *Max Planck Institute for Molecular Genetics, Berlin, Germany.ORCID http://orcid.org/0009-0003-9889-3811
Ludovic VallierMax Planck Institute for Molecular Genetics, Berlin, Germany.ORCID http://orcid.org/0000-0002-3848-2602
Helene KretzmerMax Planck Institute for Molecular Genetics, Berlin, Germany.ORCID http://orcid.org/0000-0002-0723-4980
Alexander MeissnerMax Planck Institute for Molecular Genetics, Berlin, Germany. meissner@molgen.mpg.de.ORCID http://orcid.org/0000-0001-8646-7469

Funding

European Research Council 101098178
6 · The paper itself

Abstract

Individual CpG methylation signatures characterize each mammalian cell type, thereby offering a powerful readout for clinical and diagnostic applications. Here, we apply this principle to in vitro cellular systems, using cell-free DNA released into the culture medium to track epigenomic responses to small-molecule treatments and bioreactor-based directed differentiation. Coupled with Oxford Nanopore sequencing, our approach enables rapid assessment of global CpG methylation dynamics and enhancer states. This strategy provides a streamlined method that can be readily applied and customized for non-disruptive monitoring of various in vitro screening modalities.

Indexed as

Cell-Free Nucleic AcidsDNA MethylationAnimalsBioreactorsCell DifferentiationCpG IslandsHepatocytesHumansCell-Free Nucleic AcidsBioreactorCell-free DNACell identityHepatocytesLiquid biopsyMonitoringStem cells

Identifiers

PMID41703631
PMCPMC12931013

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.