Evidence map›Paper›PMID 41703199›Full record

ArticleScientific reports2026

Hydrodistillation, comparative analysis and optimization of essential oils from Thymus schimperi R, Thymus serrulatus R, and Ocimum lamiifolium Hochst ex Benth.

Sisay Belachew Mebrate, Adane Flatie Alemu, Aychew Mekuriaw Tegegne, Minbale Gashu Tadesse, Gebremariam Ketema Bezabuh

Abstract readComparative Study
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sisay Belachew MebrateDepartment of Pharmacy, Asrat Woldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia. sisaybelachew@dbu.edu.et.
Adane Flatie AlemuSchools of Pharmacy, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopia.
Aychew Mekuriaw TegegneDepartment of Pharmacy, Asrat Woldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia.
Minbale Gashu TadesseDepartment of Chemistry, College of Natural and Computational Science, Debre Berhan University, Debre Berhan, Ethiopia.
Gebremariam Ketema BezabuhDepartment of Pharmacy, College of Medicine and Health Science, Wollo University, Dessie, Ethiopia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Essential oils are valuable natural products that are currently popular worldwide due to their positive effects on human health and their significant role in preventing and curing diseases. The increasing prevalence of antibiotic-resistant pathogens poses a major global health challenge, limiting the effectiveness of conventional antimicrobial therapies. Medicinal plants such as Thymus schimperi R., Thymus serrulatus R., and Ocimum lamiifolium Hochst. ex Benth., are traditionally used for treating infections and are known to produce essential oils rich in bioactive compounds with antimicrobial activities. Investigating the essential oils from these plants provides a potential alternative. This study focused on the parametric optimization of essential oil extraction from the leaves of T. schimperi R., T. serrulatus R., and O. lamiifolium Hochst. ex Benth., collected from the Ankober and Tarmaber Districts, North Shewa Zone, Amhara Region, Ethiopia, using hydrodistillation method. T. schimperi R., T. serrulatus R., and O. lamiifolium Hochst. ex Benth., leaves were gathered, authenticated, dried, and ground into three different particle sizes (0.25, 2, and 4 mm). Using a Clevenger apparatus, essential oils were hydrodistilled at 95 °C for specified extraction durations (30, 60, and 120 min). Particle size and extraction time were optimized using a Taguchi experimental design, and oil yield was determined gravimetrically. Every experiment was conducted in triplicate, and factors influencing yield were evaluated for significance using ANOVA (p < 0.05). The maximum yield of essential oil was obtained from T. schimperi R., (3.05%, w/w) with particle size of 2 mm and extraction time of 60 min at constant sample loading (10 g) and volume of water (350 mL). Of the parameters investigated, particle size had the most significant influence on essential oil yield. This study indicates that while pure and sufficient amounts of essential oils extracted within a short timeframe, the yield will eventually decrease due to evaporation of the extracted oils.

Indexed as

DistillationOcimumOils, VolatilePlant OilsThymus PlantEthiopiaPlant LeavesOils, VolatilePlant OilsHydrodistillationO. lamiifolium Hochst. ex Benth.Taguchi methodT. schimperi R.T.serrulatus R.

Identifiers

PMID41703199
PMCPMC13002978

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.