Evidence map›Paper›PMID 41702921›Full record

ArticleNature communications2026

Structural and mechanistic insights into azetidine-associated αKG-NHFe enzyme OkaE with multifunctional catalysis.

Xinye Wang, Junjie Yu, Tonghai Liu, Xuan Zhang, Mengxi Ju, Zhekai Xie, Nathchar Naowarojna, Linlu Ping, Yaohong Dong, Biyu Gong and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Xinye Wang *State Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Junjie Yu *State Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.ORCID http://orcid.org/0000-0002-9350-3074
Tonghai Liu *School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.ORCID http://orcid.org/0009-0003-9269-861X
Xuan Zhang *School of Pharmaceutical Sciences, Hainan University, Haikou, China.
Mengxi JuSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong Province, China.
Zhekai XieSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong Province, China.
Nathchar NaowarojnaFaculty of Science and Technology, Sakon Nakhon Rajabhat University, Sakon Nakhon, Thailand.ORCID http://orcid.org/0000-0002-7952-3458
Linlu PingState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Yaohong DongState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Biyu GongState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Yongtao XieState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Yao NieSchool of Biotechnology and Key Laboratory of Industrial Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, China.ORCID http://orcid.org/0000-0001-8065-7640
Tom HsiangEnvironmental Sciences, University of Guelph, Guelph, ON, Canada.
Ruibo WuSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong Province, China.ORCID http://orcid.org/0000-0002-1984-046X
Lixin ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Pinghua LiuDepartment of Chemistry, Boston University, Boston, MA, USA.
Guoliang ZhuState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China. zhuguoliang@ecust.edu.cn.ORCID http://orcid.org/0000-0002-6628-8874
Wupeng YanSchool of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China. yanwupeng@sjtu.edu.cn.ORCID http://orcid.org/0000-0002-0210-9734
Xueting LiuState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China. liuxueting@ecust.edu.cn.ORCID http://orcid.org/0000-0002-1322-8253

Funding

Alkaloid Biosynthetic StudiesR01GM140040 · NIGMS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI LIU, PINGHUA · 2021 to 2024
$1.4M
National Natural Science Foundation of China (National Science Foundation of China) 22307037NIGMS NIH HHS R01 GM140040
6 · The paper itself

Abstract

α-Ketoglutarate-dependent mononuclear non-haem iron (αKG-NHFe) enzymes are catalytically versatile, yet OkaE is unique for synthesizing azetidine rings via C-C bond formation. Here, we report the unexpected multifunctionality of OkaE, which catalyzes sequential oxidations. Isotopic labelling studies demonstrate that a second O₂ molecule participates in sequential epoxidation and ring cleavage, incorporating two oxygen atoms within a single catalytic cycle to form the previously unknown structure, neuokaramine IV. Crystal structures of the OkaE•Co

Indexed as

AzetidinesKetoglutaric AcidsNonheme Iron ProteinsBiocatalysisCatalysisCrystallography, X-RayModels, MolecularOxidation-ReductionOxygenazetidineAzetidinesKetoglutaric AcidsNonheme Iron ProteinsOxygen

Identifiers

PMID41702921
PMCPMC13022304

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.