Evidence map›Paper›PMID 41702344›Full record

ArticlePoultry science2026

A multiplex TaqMan real-time PCR assay for differential identification of wild-type and vaccine strains of Mycoplasma gallisepticum.

Jialiang Xin, Jiangtao Zhang, Sulian Liang, Jingshan Bi, Jianhao Wu, Zhengji Wei, Rong Yang, Zhongyi Zhao, Jing Huang, Wenjing Li and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Jialiang XinGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China; College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Jiangtao ZhangGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Sulian LiangGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China; Tiandong Center for Animal Disease Control and Prevention, Tiandong 531500, China.
Jingshan BiGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Jianhao WuGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Zhengji WeiGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Rong YangGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Zhongyi ZhaoGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Jing HuangCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Wenjing LiCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China; Guangxi Agricultureal Engineering Vocational Technical College, Fusui, 530028.
Mengjia YangCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Haotian XueCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Hao HuangGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Jing MuCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Jiafeng DingCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China. Electronic address: jiafengding@cau.edu.cn.
Lei LeiCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China. Electronic address: leilei@cau.edu.cn.
Min ZhengGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China. Electronic address: zhgmn26@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycoplasma gallisepticum (MG) is a major avian respiratory pathogen responsible for chronic respiratory disease and substantial economic losses in the poultry industry worldwide. Live attenuated vaccines, including strains F, ts-11, and 6/85, are widely used for MG control; however, current diagnostic methods are unable to reliably differentiate wild-type MG infections from vaccine strain persistence or replication, complicating surveillance and control programs in vaccinated flocks. In this study, a rapid and discriminatory multiplex TaqMan real-time quantitative PCR (qPCR) assay was developed to simultaneously detect MG and differentiate wild-type strains from the three major commercial live vaccine strains (F, ts-11, and 6/85) in a single reaction. Strain- and species-specific target regions were identified through comparative genomic analysis of 19 MG genomes, and corresponding primers and probes were designed and optimized. Under optimal conditions, the assay exhibited excellent linearity (R

Indexed as

Bacterial VaccinesChickensMultiplex Polymerase Chain ReactionMycoplasma gallisepticumMycoplasma InfectionsPoultry DiseasesReal-Time Polymerase Chain ReactionAnimalsSensitivity and SpecificityVaccines, AttenuatedBacterial VaccinesVaccines, AttenuatedmultiplexMycoplasma gallisepticumpoultryTaqManvaccine strains

Identifiers

PMID41702344
PMCPMC12925533

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.