The amplification of bacteriophages using Salmonella YB1 as a host and its therapeutic efficacy against salmonellosis in broilers.

Zhichao Hu, Jinhui Kang, Nanyan Lin, Huifang Chen, Fang Liu, Juan Li, Cui Zhu, Yinshan Bai

Abstract read

In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhichao HuGuangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong, PR China; Key Laboratory of Livestock Disease Prevention of Guangdong Province; Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, PR China.

Jinhui KangGuangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong, PR China.

Nanyan LinGuangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong, PR China.

Huifang ChenGuangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong, PR China.

Fang LiuGuangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong, PR China.

Juan LiKey Laboratory of Livestock Disease Prevention of Guangdong Province; Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, PR China.

Cui ZhuGuangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong, PR China.

Yinshan BaiGuangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong, PR China. Electronic address: baiyinshan@fosu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The residual bacteria in bacteriophage (phage) preparations have hindered their application in poultry disease. We isolated eight phages from aquatic environment samples and selected two phages (vB_StyS_SP03 and vB_StyM_SP07) for amplification using the diaminopimelic acid (DAP)-dependent Salmonella YB1 strain. Both healthy and Salmonella-infected broilers were intraperitoneally injected with phages amplified by Salmonella YB1 and their cocktail to evaluate the safety and therapeutic efficacy. The results showed that Salmonella YB1 effectively amplified phages, whose form and function remained unchanged after amplification. Salmonella YB1 in the phage preparations did not proliferate without DAP supplementation. Phage administration caused no mortality, and did not significantly affect the weight gain, immune organ indexes, and gut microbiota composition in healthy broilers. In Salmonella-challenged broilers, phage administration significantly improved weight gain, reduced organ indexes, alleviated clinical symptoms and pathological changes, and restored the intestinal microbial community. Collectively, we have developed a method for amplifying phages using Salmonella YB1, which effectively addresses the problem of residual bacteria in phage preparations.

Indexed as

ChickensPoultry DiseasesSalmonellaSalmonella Infections, AnimalSalmonella PhagesAnimalsAntibioticBroilerPhage amplificationSalmonella TyphimuriumSalmonella YB1

Identifiers

PMID41702339

DOI10.1016/j.psj.2026.106610

PMCPMC12925554

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.