Evidence map›Paper›PMID 41701574›Full record

ArticleSTAR protocols2026

Protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes.

Ankur Kumar, Hua Bai

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Ankur KumarInterdisciplinary Genetics and Genomics Program, Iowa State University, Ames, IA 50011, USA; Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA. Electronic address: ankurk@iastate.edu.
Hua BaiInterdisciplinary Genetics and Genomics Program, Iowa State University, Ames, IA 50011, USA; Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA. Electronic address: hbai@iastate.edu.

Funding

Autonomous and Non-Autonomous Regulation of Cardiac AgingR01AG058741 · NIA · IOWA STATE UNIVERSITY · PI BAI, HUA · 2019 to 2023
$1.5M
Role of peroxisome-mitochondrion communication in tissue agingR01AG075156 · NIA · IOWA STATE UNIVERSITY · PI Hua Bai · 2023 to 2026
$1.2M
NIA NIH HHS R01 AG058741NIA NIH HHS R01 AG075156
6 · The paper itself

Abstract

Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations.

Indexed as

DrosophilaMitochondrial DynamicsAnimalsDrosophila melanogasterMicroscopy, ConfocalMitochondriaTime-Lapse ImagingCell BiologyGeneticsMicroscopyModel Organisms

Identifiers

PMID41701574
PMCPMC12925548

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.