Evidence map›Paper›PMID 41701573›Full record

ArticleSTAR protocols2026

Protocol for isolation of total RNA from mouse whole cochlea using organic solvents.

Ezequiel Rías, Guillermo Spitzmaul, Leonardo Dionisio

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ezequiel RíasInstituto de Investigaciones Bioquímicas de Bahía Blanca, CONICET-UNS, Bahía Blanca 8000, Argentina; Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur (UNS), Bahía Blanca 8000, Argentina.
Guillermo SpitzmaulInstituto de Investigaciones Bioquímicas de Bahía Blanca, CONICET-UNS, Bahía Blanca 8000, Argentina; Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur (UNS), Bahía Blanca 8000, Argentina.
Leonardo DionisioInstituto de Investigaciones Bioquímicas de Bahía Blanca, CONICET-UNS, Bahía Blanca 8000, Argentina; Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur (UNS), Bahía Blanca 8000, Argentina. Electronic address: ldionisio@inibibb-conicet.gob.ar.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Determining gene expression changes in cochlear components is important for understanding the mechanisms underlying hearing loss. Here, we present a protocol for the isolation of total RNA from mouse cochleae across a wide range of ages (2-25 weeks old) using organic solvent extraction. We describe the steps for obtaining the entire cochlea from mice and isolating high-quality total RNA suitable for further reverse transcription and PCR. For complete details on the use and execution of this protocol, please refer to Rías et al.

Indexed as

CochleaRNASolventsAnimalsMiceRNASolventsGene ExpressionGeneticsModel OrganismsSystems biology

Identifiers

PMID41701573
PMCPMC12924179

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.